Hotspot

ycf1

SSC · 126133-131766 · 5634 bp

Back to Glyptopetalum

Pi 0.0168

MarkerSeek score 57.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5634
variable_sites: 94
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGAAATGGGCGATTCCATC	TGATGAATCCGACTTTCCATTGA	50.0	39.1	58.4	58.2	169-193 bp	1.0	57.7
ycf1_p2	TCGAAATGGGCGATTCCATC	TGATGAATCCGACTTTCCATTG	50.0	40.9	58.4	56.8	169-193 bp	1.0	57.1
ycf1_p3	TCGAAATGGGCGATTCCATC	TGAATCCGACTTTCCATTGA	50.0	40.0	58.4	54.7	166-190 bp	1.0	56.3
ycf1_p4	TCGAAATGGGCGATTCCATC	TGAATCCGACTTTCCATTGAA	50.0	38.1	58.4	55.5	166-190 bp	1.0	55.0
ycf1_p5	TCGAAATGGGCGATTCCATC	GATGAATCCGACTTTCCATTGA	50.0	40.9	58.4	56.5	168-192 bp	1.0	55.0