Hotspot

rps15

SSC · 125452-125730 · 279 bp

Back to Glyptopetalum

Pi 0.0179
MarkerSeek score 49.00
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
279
variable_sites
5
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 AGGTAGATCGGCCACTACTC AACCTGTGGTAACGTTTCGA 55.0 45.0 58.0 57.4 1506-1512 bp 1.0 76.9
rps15_p2 AGGTAGATCGGCCACTACTC ACCTGTGGTAACGTTTCGAA 55.0 45.0 58.0 57.4 1505-1511 bp 1.0 76.9
rps15_p3 GAGGTAGATCGGCCACTACT ACCTGTGGTAACGTTTCGAA 55.0 45.0 58.0 57.4 1506-1512 bp 1.0 76.9
rps15_p4 GTAGATCGGCCACTACTCCT AACCTGTGGTAACGTTTCGA 55.0 45.0 58.0 57.4 1504-1510 bp 1.0 76.9
rps15_p5 GTAGATCGGCCACTACTCCT ACCTGTGGTAACGTTTCGAA 55.0 45.0 58.0 57.4 1503-1509 bp 1.0 76.9