Hotspot

accD

LSC · 59261-60760 · 1500 bp

Back to Glyptopetalum

Pi 0.0175
MarkerSeek score 60.40
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1500
variable_sites
26
pi
0.0175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 TCATGTAAATAAGGGCAAGAAAGT AGGTTTCCTGGTTAGTAATCAGG 33.3 43.5 56.6 57.6 1581-1599 bp 1.0 42.1
accD_p2 TGTAAATAAGGGCAAGAAAGTTCT AGGTTTCCTGGTTAGTAATCAGG 33.3 43.5 56.5 57.6 1578-1596 bp 1.0 42.1
accD_p3 AATAAGGGCAAGAAAGTTCT AGGTTTCCTGGTTAGTAATCAGG 35.0 43.5 52.3 57.6 1574-1592 bp 1.0 42.1
accD_p4 TCAGTGAATTTTTCATCGAATGACT AGGTTTCCTGGTTAGTAATCAGG 32.0 43.5 57.6 57.6 1628-1646 bp 1.0 42.0
accD_p5 TCAGTGAATTTTTCATCGAATGAC AGGTTTCCTGGTTAGTAATCAGG 33.3 43.5 56.3 57.6 1628-1646 bp 1.0 42.0