Hotspot

ccsA-ndhD

SSC · 117446-117697 · 252 bp

Back to Glyptopetalum

Pi 0.0585

MarkerSeek score 69.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 252
variable_sites: 12
pi: 0.0585
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TAGGAATAGGGCTGCACAGT	GTGACAAGGTCGAGGCTATT	50.0	50.0	58.1	57.3	290-337 bp	1.0	80.7
ccsA-ndhD_p2	GGCTGCACAGTTATGGTTCA	GTGACAAGGTCGAGGCTATT	50.0	50.0	58.5	57.3	281-328 bp	1.0	78.0
ccsA-ndhD_p3	TAGGAATAGGGCTGCACAGT	GTGACAAGGTCGAGGCTATTT	50.0	47.6	58.1	58.0	290-337 bp	1.0	77.9
ccsA-ndhD_p4	TAGGAATAGGGCTGCACAGT	GGTGACAAGGTCGAGGCTAT	50.0	55.0	58.1	59.2	291-338 bp	1.0	76.7
ccsA-ndhD_p5	ATAGGGCTGCACAGTTATGG	GTGACAAGGTCGAGGCTATT	50.0	50.0	57.4	57.3	285-332 bp	1.0	76.5