Hotspot

trnL-UAG-ccsA

SSC · 116386-116455 · 70 bp

Back to Glyptopetalum

Pi 0.0429

MarkerSeek score 77.10

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 70
variable_sites: 3
pi: 0.0429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	TGGAAACAACCGAAACGGAT	50.0	45.0	58.0	58.0	217 bp	1.0	80.8
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	TGGAAACAACCGAAACGGAT	50.0	45.0	58.0	58.0	216 bp	1.0	80.8
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	TGGAAACAACCGAAACGGAT	50.0	45.0	57.9	58.0	207 bp	1.0	79.9
trnL-UAG-ccsA_p4	GGTAGACACGCTGCTCTTAG	TGGAAACAACCGAAACGGAT	55.0	45.0	57.8	58.0	209 bp	1.0	79.3
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	TGGAAACAACCGAAACGGAT	55.0	45.0	57.8	58.0	208 bp	1.0	79.3