Hotspot

rpl22-rps19

LSC · 85639-85751 · 113 bp

Back to Glyptopetalum

Pi 0.0617
MarkerSeek score 73.10
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
113
variable_sites
5
pi
0.0617
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.72
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 CCTCGTAAAAACGTCCACGA AAATTTCCGGGGACATGCAA 50.0 45.0 58.2 58.4 274-304 bp 1.0 80.7
rpl22-rps19_p2 CCTCGTAAAAACGTCCACGA AATTTCCGGGGACATGCAAA 50.0 45.0 58.2 58.4 273-303 bp 1.0 80.7
rpl22-rps19_p3 CCTCGTAAAAACGTCCACGA ATTTCCGGGGACATGCAAAA 50.0 45.0 58.2 58.4 272-302 bp 1.0 80.7
rpl22-rps19_p4 TCCTCGTAAAAACGTCCACG AATTTCCGGGGACATGCAAA 50.0 45.0 58.2 58.4 274-304 bp 1.0 80.7
rpl22-rps19_p5 TCCTCGTAAAAACGTCCACG ATTTCCGGGGACATGCAAAA 50.0 45.0 58.2 58.4 273-303 bp 1.0 80.7