Hotspot

psbZ-trnG-GCC

LSC · 36985-37725 · 741 bp

Back to Glyptopetalum

Pi 0.0419

MarkerSeek score 75.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 27
pi: 0.0419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	856-899 bp	1.0	82.9
psbZ-trnG-GCC_p2	TGAGGGTTGGTCGAGTAACA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	845-888 bp	1.0	82.8
psbZ-trnG-GCC_p3	CTGAGGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	57.6	57.8	846-889 bp	1.0	82.2
psbZ-trnG-GCC_p4	CCTGAGGGTTGGTCGAGTAA	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.7	57.8	847-890 bp	1.0	79.2
psbZ-trnG-GCC_p5	GAGGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	844-887 bp	1.0	78.5