Hotspot

psbC-trnS-UGA

LSC · 36094-36355 · 262 bp

Back to Glyptopetalum

Pi 0.0349

MarkerSeek score 72.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 262
variable_sites: 9
pi: 0.0349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TTGATCGCGATTTTGAGCCT	AGCTCTGGTCTTGAAAACCG	45.0	50.0	58.3	58.1	375-379 bp	1.0	79.7
psbC-trnS-UGA_p2	TTGATCGCGATTTTGAGCCT	GATGGCCGAGTGGTTGATAG	45.0	55.0	58.3	58.1	393-397 bp	1.0	79.5
psbC-trnS-UGA_p3	GCTGCGGGATTTGAAAAAGG	AGCTCTGGTCTTGAAAACCG	50.0	50.0	58.3	58.1	397-401 bp	1.0	79.4
psbC-trnS-UGA_p4	GCTGCGGGATTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.3	58.1	415-419 bp	1.0	79.2
psbC-trnS-UGA_p5	TTGATCGCGATTTTGAGCCT	CGAGTGGTTGATAGCTCTGG	45.0	55.0	58.3	57.8	387-391 bp	1.0	78.8