Hotspot

trnD-GUC-trnY-GUA

LSC · 30871-31318 · 448 bp

Back to Glyptopetalum

Pi 0.0184

MarkerSeek score 55.30

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 8
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	AGGATCCCTTGGGAATGACA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	676 bp	0.5	58.0
trnD-GUC-trnY-GUA_p2	AGGATCCCTTGGGAATGACA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	675 bp	0.5	58.0
trnD-GUC-trnY-GUA_p3	AGGATCCCTTGGGAATGACA	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	58.0	58.3	831 bp	0.5	57.8
trnD-GUC-trnY-GUA_p4	AGGATCCCTTGGGAATGACA	CAAATATGCCCCCATCGTCT	50.0	50.0	58.0	57.7	866 bp	0.5	57.7
trnD-GUC-trnY-GUA_p5	AGGATCCCTTGGGAATGACA	TCGTCTAGTGGTTCAGGACA	50.0	50.0	58.0	57.7	852 bp	0.5	57.6