Hotspot

psbM-trnD-GUC

LSC · 30335-30796 · 462 bp

Back to Glyptopetalum

Pi 0.0814
MarkerSeek score 81.40
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
462
variable_sites
35
pi
0.0814
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 GCAAAAAGGCAGTAGGAACT GTTCGAGCCCCGTCAGTC 45.0 66.7 56.2 60.1 550-557 bp 1.0 49.1
psbM-trnD-GUC_p2 AGCAAAAAGGCAGTAGGAACT GTTCGAGCCCCGTCAGTC 42.9 66.7 57.8 60.1 551-558 bp 1.0 49.0
psbM-trnD-GUC_p3 AGCAAAAAGGCAGTAGGAAC GTTCGAGCCCCGTCAGTC 45.0 66.7 56.2 60.1 551-558 bp 1.0 49.0
psbM-trnD-GUC_p4 AGCAAAAAGGCAGTAGGAACT GAGCACCGCCCTGTCAAG 42.9 66.7 57.8 60.7 582-589 bp 1.0 48.8
psbM-trnD-GUC_p5 AGCAAAAAGGCAGTAGGAACT GCCCTGTCAAGGCGGAAG 42.9 66.7 57.8 60.7 575-582 bp 1.0 48.8