Hotspot

psbI-trnS-GCU

LSC · 7507-7738 · 232 bp

Back to Glyptopetalum

Pi 0.0354

MarkerSeek score 74.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 232
variable_sites: 8
pi: 0.0354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCCCGGACGCGAAGAATAA	GTTCTTCGTACCGAGGGTTC	50.0	55.0	59.3	58.0	289-306 bp	1.0	73.7
psbI-trnS-GCU_p2	AATCCCGGACGCGAAGAATA	TGGACTAAAGCGTCGGATTG	50.0	50.0	59.3	58.0	326-343 bp	1.0	73.3
psbI-trnS-GCU_p3	ATCCCGGACGCGAAGAATAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	59.3	58.0	325-342 bp	1.0	73.3
psbI-trnS-GCU_p4	AATCCCGGACGCGAAGAATA	GTGGACTAAAGCGTCGGATT	50.0	50.0	59.3	58.0	327-344 bp	1.0	73.3
psbI-trnS-GCU_p5	ATCCCGGACGCGAAGAATAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	59.3	58.0	326-343 bp	1.0	73.3