Hotspot

rps16-trnQ-UUG

LSC · 5253-6281 · 1029 bp

Back to Glyptopetalum

Pi 0.0219
MarkerSeek score 67.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1029
variable_sites
22
pi
0.0219
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TTTCCTTCTCTTCGCGATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 1142-1145 bp 1.0 82.0
rps16-trnQ-UUG_p2 GGTTTTCCTTCTCTTCGCGA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1145-1148 bp 1.0 81.0
rps16-trnQ-UUG_p3 TTTCCTTCTCTTCGCGATCG AATAGAATATGGGGCGTGGC 50.0 50.0 58.1 57.8 1199-1202 bp 1.0 80.8
rps16-trnQ-UUG_p4 GGTTTTCCTTCTCTTCGCGA AATAGAATATGGGGCGTGGC 50.0 50.0 58.2 57.8 1202-1205 bp 1.0 79.9
rps16-trnQ-UUG_p5 GCGATCGAACATCAATTGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.5 58.1 1129-1132 bp 1.0 79.1