Hotspot

trnK-UUU-rps16

LSC · 4370-4952 · 583 bp

Back to Glyptopetalum

Pi 0.0312

MarkerSeek score 62.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 18
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GCATTTTGTTTTGCGCCAAG	CGATCGCGAAGAGAAGGAAA	45.0	50.0	58.0	58.1	1047 bp	1.0	81.3
trnK-UUU-rps16_p2	GCATTTTGTTTTGCGCCAAG	TTAGAACCCCCAACCCTCAT	45.0	50.0	58.0	57.9	1404 bp	1.0	81.2
trnK-UUU-rps16_p3	CCTTCATCTCCACCCCCTAA	CGATCGCGAAGAGAAGGAAA	55.0	50.0	58.1	58.1	1303-1306 bp	1.0	80.7
trnK-UUU-rps16_p4	CCTTCATCTCCACCCCCTAA	TTAGAACCCCCAACCCTCAT	55.0	50.0	58.1	57.9	1660-1663 bp	1.0	80.6
trnK-UUU-rps16_p5	GCATTTTGTTTTGCGCCAAG	TTTAGAACCCCCAACCCTCA	45.0	50.0	58.0	58.2	1405 bp	1.0	80.5