Hotspot

trnH-GUG-psbA

LSC · 61-450 · 390 bp

Back to Glyptopetalum

Pi 0.0449

MarkerSeek score 82.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 390
variable_sites: 17
pi: 0.0449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	507-634 bp	1.0	82.6
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	521-648 bp	1.0	82.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	520-647 bp	1.0	82.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	522-649 bp	1.0	82.5
trnH-GUG-psbA_p5	GATCCACAATCCACTGCCTT	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	527-654 bp	1.0	82.5