Hotspot

trnR-ACG-trnN-GUU

Genome · 107052-107592 · 541 bp

Back to Glycyrrhiza

Pi 0.0199

MarkerSeek score 63.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: Genome
length_bp: 541
variable_sites: 52
pi: 0.0199
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCAAGTCCT	55.0	50.0	57.8	58.0	80-638 bp	1.0	87.4
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.8	57.7	80-681 bp	1.0	84.4
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	CCGATTGGTCGTAGGTTCAA	55.0	50.0	57.8	57.6	80-643 bp	1.0	84.4
trnR-ACG-trnN-GUU_p4	GGCCTGTAGCTCAGAGGATT	TGGTCGTAGGTTCAAGTCCT	55.0	50.0	58.9	58.0	80-649 bp	1.0	82.0
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	GTTCCTCAGTAGCTCAGTGG	55.0	55.0	57.8	57.3	80-682 bp	1.0	81.6