Hotspot

rps12-trnV-GAC

Genome · 97655-99314 · 1660 bp

Back to Glycyrrhiza

Pi 0.00689
MarkerSeek score 63.20
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
Genome
length_bp
1660
variable_sites
66
pi
0.00689
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 GAGGTTAAGCGTACTCTGGC ATTCGATCCCGGCATCAAAA 55.0 45.0 58.1 57.9 80-1901 bp 1.0 85.8
rps12-trnV-GAC_p2 GAGGTTAAGCGTACTCTGGC TTCGATCCCGGCATCAAAAT 55.0 45.0 58.1 57.9 80-1900 bp 1.0 85.8
rps12-trnV-GAC_p3 GGTTAAGCGTACTCTGGCAA ATTCGATCCCGGCATCAAAA 50.0 45.0 57.9 57.9 80-1899 bp 1.0 85.7
rps12-trnV-GAC_p4 GGTTAAGCGTACTCTGGCAA TTCGATCCCGGCATCAAAAT 50.0 45.0 57.9 57.9 80-1898 bp 1.0 85.7
rps12-trnV-GAC_p5 GAGGTTAAGCGTACTCTGGC AATTCTCATTCGATCCCGGC 55.0 50.0 58.1 58.1 80-1908 bp 0.961538 83.0