Hotspot

petA-psbJ

Genome · 63244-64346 · 1103 bp

Back to Glycyrrhiza

Pi 0.00927

MarkerSeek score 54.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 1103
variable_sites: 63
pi: 0.00927
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	ACAGGTATTGTCGTGATCGG	42.9	50.0	57.2	57.4	1187-1262 bp	1.0	69.5
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGA	CAGGTATTGTCGTGATCGGT	42.9	50.0	57.2	57.4	1186-1261 bp	1.0	69.5
petA-psbJ_p3	TGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGATCATCC	42.9	50.0	57.2	57.1	1138-1213 bp	1.0	67.2
petA-psbJ_p4	TGAGAAGGTTCAATTGTCCGAA	ACAGGTATTGTCGTGATCGG	40.9	50.0	57.9	57.4	1187-1262 bp	1.0	66.9
petA-psbJ_p5	TGAGAAGGTTCAATTGTCCGAA	CAGGTATTGTCGTGATCGGT	40.9	50.0	57.9	57.4	1186-1261 bp	1.0	66.9