Hotspot

trnQ-UUG-accD

Genome · 56940-58119 · 1180 bp

Back to Glycyrrhiza

Pi 0.0134

MarkerSeek score 60.00

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: Genome
length_bp: 1180
variable_sites: 83
pi: 0.0134
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	ACAGCATAGAATTGAACCACCA	55.0	40.9	58.1	58.0	1162-1290 bp	1.0	72.5
trnQ-UUG-accD_p2	TTCGGAGGTTCGAATCCTTC	ACAGCATAGAATTGAACCACCA	50.0	40.9	57.3	58.0	1166-1294 bp	1.0	67.8
trnQ-UUG-accD_p3	GAGGTTCGAATCCTTCCGTC	CAGCATAGAATTGAACCACCA	55.0	42.9	58.1	56.2	1161-1289 bp	1.0	66.6
trnQ-UUG-accD_p4	GAGGTTCGAATCCTTCCGTC	ACAGCATAGAATTGAACCACC	55.0	42.9	58.1	56.2	1162-1290 bp	1.0	66.6
trnQ-UUG-accD_p5	GGTTTTGGTCCCGTTATTCG	ACAGCATAGAATTGAACCACCA	50.0	40.9	57.1	58.0	1182-1310 bp	1.0	65.8