Hotspot

atpI-atpH

Genome · 48509-49508 · 1000 bp

Back to Glycyrrhiza

Pi 0.00815

MarkerSeek score 65.50

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 1000
variable_sites: 46
pi: 0.00815
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	CGCAATCCCTTCTACAGCTT	50.0	50.0	57.3	58.0	1098-1182 bp	1.0	81.1
atpI-atpH_p2	ACAAGCGGTATTCAAGCTCT	CGCAATCCCTTCTACAGCTT	45.0	50.0	57.2	58.0	1134-1218 bp	1.0	80.7
atpI-atpH_p3	GCTGCGGCTTATATAGGTGA	AACAGAAGCGGCAGAAATCA	50.0	45.0	57.3	58.1	1008-1092 bp	1.0	80.7
atpI-atpH_p4	GGTGAATCCATGGAAGGTCA	CGCAATCCCTTCTACAGCTT	50.0	50.0	57.2	58.0	1083-1167 bp	1.0	80.6
atpI-atpH_p5	GCTGCGGCTTATATAGGTGA	GTCCAATAGAAGCAAGCCCA	50.0	50.0	57.3	57.9	1048-1132 bp	1.0	80.3