Hotspot

trnF-GAA-trnL-UAA

Genome · 13497-13864 · 368 bp

Back to Glycyrrhiza

Pi 0.0125

MarkerSeek score 53.10

Primer available yes

Label trnF-GAA-trnL-UAA

Feature Metadata

label_name: trnF-GAA-trnL-UAA
feature_type: igs
region: Genome
length_bp: 368
variable_sites: 18
pi: 0.0125
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnL-UAA_p1	TTTGAACTGGTGACACGAGG	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.1	57.5	447-463 bp	1.0	82.8
trnF-GAA-trnL-UAA_p2	AAATGAATCATGTGCCGGGA	AATCGTGAGGGTTCAAGTCC	45.0	50.0	57.9	57.5	473-489 bp	1.0	81.7
trnF-GAA-trnL-UAA_p3	AATGAATCATGTGCCGGGAA	AATCGTGAGGGTTCAAGTCC	45.0	50.0	57.9	57.5	472-488 bp	1.0	81.7
trnF-GAA-trnL-UAA_p4	CAAATGAATCATGTGCCGGG	AATCGTGAGGGTTCAAGTCC	50.0	50.0	57.8	57.5	474-490 bp	1.0	81.1
trnF-GAA-trnL-UAA_p5	TACCAACTGAGCTATCCCGA	AATCGTGAGGGTTCAAGTCC	50.0	50.0	57.6	57.5	409-425 bp	1.0	80.1