Hotspot

trnK-UUU-rbcL

Genome · 4372-5450 · 1079 bp

Back to Glycyrrhiza

Pi 0.0102

MarkerSeek score 51.30

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: Genome
length_bp: 1079
variable_sites: 43
pi: 0.0102
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	TGGAGTCGCACTTAAAAGCC	TTTGAATTCCCGGCAATGGA	50.0	45.0	58.5	58.1	1131-1220 bp	1.0	82.5
trnK-UUU-rbcL_p2	AACCCTACCGATGGTATGGA	TTTGAATTCCCGGCAATGGA	50.0	45.0	57.5	58.1	1184-1273 bp	1.0	82.4
trnK-UUU-rbcL_p3	AGTCGTGGTCTTACAAACCC	TTTGAATTCCCGGCAATGGA	50.0	45.0	57.5	58.1	1199-1288 bp	1.0	81.9
trnK-UUU-rbcL_p4	GTCGTGGTCTTACAAACCCT	TTTGAATTCCCGGCAATGGA	50.0	45.0	57.5	58.1	1198-1287 bp	1.0	81.9
trnK-UUU-rbcL_p5	TGGAGTCGCACTTAAAAGCC	TTGAATTCCCGGCAATGGAT	50.0	45.0	58.5	57.8	1130-1219 bp	1.0	81.8