primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	trnK-UUU-rbcL	1	TGGAGTCGCACTTAAAAGCC	TTTGAATTCCCGGCAATGGA	20	20	50.0	45.0	58.5	58.1	0.0	2.3	0.550341	4372	5450	1131-1220	1.000000	82.5
trnK-UUU-rbcL_p2	trnK-UUU-rbcL	2	AACCCTACCGATGGTATGGA	TTTGAATTCCCGGCAATGGA	20	20	50.0	45.0	57.5	58.1	20.0	2.3	0.552164	4372	5450	1184-1273	1.000000	82.4
trnK-UUU-rbcL_p3	trnK-UUU-rbcL	3	AGTCGTGGTCTTACAAACCC	TTTGAATTCCCGGCAATGGA	20	20	50.0	45.0	57.5	58.1	0.0	2.3	0.608261	4372	5450	1199-1288	1.000000	81.9
trnK-UUU-rbcL_p4	trnK-UUU-rbcL	4	GTCGTGGTCTTACAAACCCT	TTTGAATTCCCGGCAATGGA	20	20	50.0	45.0	57.5	58.1	0.0	2.3	0.608261	4372	5450	1198-1287	1.000000	81.9
trnK-UUU-rbcL_p5	trnK-UUU-rbcL	5	TGGAGTCGCACTTAAAAGCC	TTGAATTCCCGGCAATGGAT	20	20	50.0	45.0	58.5	57.8	0.0	2.3	0.638231	4372	5450	1130-1219	1.000000	81.8
trnV-UAC-ndhC_p1	trnV-UAC-ndhC	1	AGAAGGTCTACGGTTCGAGT	TTCTGGCCCTTTGCTAATCG	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.447090	10570	11006	587-653	0.961538	81.7
trnV-UAC-ndhC_p2	trnV-UAC-ndhC	2	GAAGGTCTACGGTTCGAGTC	TTCTGGCCCTTTGCTAATCG	20	20	55.0	50.0	57.5	58.2	0.0	0.0	0.790862	10570	11006	586-652	0.961538	79.0
trnV-UAC-ndhC_p3	trnV-UAC-ndhC	3	GTTCGAGTCCGTATAGCCCT	TTCTGGCCCTTTGCTAATCG	20	20	55.0	50.0	58.7	58.2	0.0	0.0	0.933156	10570	11006	575-641	0.961538	78.0
trnV-UAC-ndhC_p4	trnV-UAC-ndhC	4	AAGGTCTACGGTTCGAGTCC	TTCTGGCCCTTTGCTAATCG	20	20	55.0	50.0	58.8	58.2	0.0	0.0	1.077632	10570	11006	585-651	0.961538	76.7
trnV-UAC-ndhC_p5	trnV-UAC-ndhC	5	GAGAAGGTCTACGGTTCGAG	TTCTGGCCCTTTGCTAATCG	20	20	55.0	50.0	57.2	58.2	0.0	0.0	1.078681	10570	11006	588-654	0.961538	76.7
trnF-GAA-trnL-UAA_p1	trnF-GAA-trnL-UAA	1	TTTGAACTGGTGACACGAGG	AATCGTGAGGGTTCAAGTCC	20	20	50.0	50.0	58.1	57.5	20.1	0.0	0.526305	13497	13864	447-463	1.000000	82.8
trnF-GAA-trnL-UAA_p2	trnF-GAA-trnL-UAA	2	AAATGAATCATGTGCCGGGA	AATCGTGAGGGTTCAAGTCC	20	20	45.0	50.0	57.9	57.5	2.2	0.0	0.616766	13497	13864	473-489	1.000000	81.7
trnF-GAA-trnL-UAA_p3	trnF-GAA-trnL-UAA	3	AATGAATCATGTGCCGGGAA	AATCGTGAGGGTTCAAGTCC	20	20	45.0	50.0	57.9	57.5	2.2	0.0	0.616766	13497	13864	472-488	1.000000	81.7
trnF-GAA-trnL-UAA_p4	trnF-GAA-trnL-UAA	4	CAAATGAATCATGTGCCGGG	AATCGTGAGGGTTCAAGTCC	20	20	50.0	50.0	57.8	57.5	2.2	0.0	0.687279	13497	13864	474-490	1.000000	81.1
trnF-GAA-trnL-UAA_p5	trnF-GAA-trnL-UAA	5	TACCAACTGAGCTATCCCGA	AATCGTGAGGGTTCAAGTCC	20	20	50.0	50.0	57.6	57.5	0.0	0.0	0.921818	13497	13864	409-425	1.000000	80.1
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	ACCAATTTCGCCATATCCCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137364	14449	16024	1111-1766	1.000000	89.2
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	ACCAATTTCGCCATATCCCC	GAGTCGAACCGATGACCATC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.163174	14449	16024	1142-1797	1.000000	88.9
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	14449	16024	1119-1774	1.000000	88.6
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	TAGCGTCTACCAATTTCGCC	GAGTCGAACCGATGACCATC	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.227983	14449	16024	1150-1805	1.000000	88.3
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.2	57.9	10.2	0.0	0.372548	14449	16024	1125-1780	1.000000	87.3
ycf3-psaA_p1	ycf3-psaA	1	AGCCCTTACCCCTGGTAATT	TGCCCATTCCTCGAAAGAAG	20	20	50.0	50.0	58.0	57.9	6.2	4.1	0.117992	19538	20429	985-1083	1.000000	85.7
ycf3-psaA_p2	ycf3-psaA	2	AGCCCTTACCCCTGGTAATT	GCCCATTCCTCGAAAGAAGT	20	20	50.0	50.0	58.0	57.9	6.2	4.1	0.119474	19538	20429	984-1082	1.000000	85.7
ycf3-psaA_p3	ycf3-psaA	3	AGCTATAGCCCTTACCCCTG	TGCCCATTCCTCGAAAGAAG	20	20	55.0	50.0	58.0	57.9	21.2	4.1	0.145355	19538	20429	991-1089	1.000000	85.5
ycf3-psaA_p4	ycf3-psaA	4	AGCTATAGCCCTTACCCCTG	GCCCATTCCTCGAAAGAAGT	20	20	55.0	50.0	58.0	57.9	21.2	4.1	0.146838	19538	20429	990-1088	1.000000	85.5
ycf3-psaA_p5	ycf3-psaA	5	TTGGTTGAAGATCACAGGGC	TGCCCATTCCTCGAAAGAAG	20	20	50.0	50.0	58.1	57.9	0.0	4.1	0.204161	19538	20429	949-1047	1.000000	85.1
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	GAGCAATAGACCGGACCAAC	CAGTGTGGTAGAGTAACGCC	20	20	55.0	55.0	58.3	58.0	9.5	0.0	0.354679	29648	31130	1628-1703	1.000000	83.8
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	GAGCAATAGACCGGACCAAC	GTGGTAGAGTAACGCCATGG	20	20	55.0	55.0	58.3	58.1	9.5	7.6	0.410916	29648	31130	1624-1699	1.000000	83.3
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	AAGAGCAATAGACCGGACCA	CAGTGTGGTAGAGTAACGCC	20	20	50.0	55.0	58.4	58.0	9.5	0.0	0.439326	29648	31130	1630-1705	1.000000	83.1
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	AGAGCAATAGACCGGACCAA	CAGTGTGGTAGAGTAACGCC	20	20	50.0	55.0	58.4	58.0	9.5	0.0	0.439326	29648	31130	1629-1704	1.000000	83.1
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	AGAGCAATAGACCGGACCAA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.4	58.1	9.5	7.6	0.495564	29648	31130	1625-1700	1.000000	82.7
psbM-petN_p1	psbM-petN	1	TGCTACTGCACTCTTCATTCT	TCTTGCTTGGGCTGCTTTAA	21	20	42.9	45.0	57.0	58.0	0.0	0.0	1.975331	33306	33834	568-685	1.000000	71.5
psbM-petN_p2	psbM-petN	2	TGCTACTGCACTCTTCATTCT	AGTAAGTCTTGCTTGGGCTG	21	20	42.9	50.0	57.0	57.8	0.0	0.0	2.160415	33306	33834	574-691	1.000000	70.0
psbM-petN_p3	psbM-petN	3	TGCTACTGCACTCTTCATTCT	ATGGGGAAGAAGTGGACTCT	21	20	42.9	50.0	57.0	57.6	0.0	0.0	2.320150	33306	33834	514-631	1.000000	69.0
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTCTTCATTC	TCTTGCTTGGGCTGCTTTAA	20	20	45.0	45.0	55.5	58.0	0.0	0.0	2.521758	33306	33834	568-685	1.000000	67.1
psbM-petN_p5	psbM-petN	5	TGCTACTGCACTCTTCATTC	AGTAAGTCTTGCTTGGGCTG	20	20	45.0	50.0	55.5	57.8	0.0	0.0	2.706841	33306	33834	574-691	1.000000	65.6
petN-trnC-GCA_p1	petN-trnC-GCA	1	TTAAAGCAGCCCAAGCAAGA	TTTTGTATCGTTTTGGCGGC	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.059326	33925	34993	1185-1235	1.000000	87.6
petN-trnC-GCA_p2	petN-trnC-GCA	2	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.088172	33925	34993	1129-1179	1.000000	87.5
petN-trnC-GCA_p3	petN-trnC-GCA	3	CAGCCCAAGCAAGACTTACT	TTTTGTATCGTTTTGGCGGC	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.244410	33925	34993	1179-1229	1.000000	86.1
petN-trnC-GCA_p4	petN-trnC-GCA	4	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.273256	33925	34993	1123-1173	1.000000	86.0
petN-trnC-GCA_p5	petN-trnC-GCA	5	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	20	20	45.0	50.0	58.0	58.4	0.0	14.7	0.361535	33925	34993	1147-1197	1.000000	85.3
atpI-atpH_p1	atpI-atpH	1	GCTGCGGCTTATATAGGTGA	CGCAATCCCTTCTACAGCTT	20	20	50.0	50.0	57.3	58.0	11.2	0.0	0.773474	48509	49508	1098-1182	1.000000	81.1
atpI-atpH_p2	atpI-atpH	2	ACAAGCGGTATTCAAGCTCT	CGCAATCCCTTCTACAGCTT	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.800002	48509	49508	1134-1218	1.000000	80.7
atpI-atpH_p3	atpI-atpH	3	GCTGCGGCTTATATAGGTGA	AACAGAAGCGGCAGAAATCA	20	20	50.0	45.0	57.3	58.1	11.2	1.4	0.847416	48509	49508	1008-1092	1.000000	80.7
atpI-atpH_p4	atpI-atpH	4	GGTGAATCCATGGAAGGTCA	CGCAATCCCTTCTACAGCTT	20	20	50.0	50.0	57.2	58.0	20.7	0.0	0.836783	48509	49508	1083-1167	1.000000	80.6
atpI-atpH_p5	atpI-atpH	5	GCTGCGGCTTATATAGGTGA	GTCCAATAGAAGCAAGCCCA	20	20	50.0	50.0	57.3	57.9	11.2	0.0	0.872406	48509	49508	1048-1132	1.000000	80.3
trnQ-UUG-accD_p1	trnQ-UUG-accD	1	GAGGTTCGAATCCTTCCGTC	ACAGCATAGAATTGAACCACCA	20	22	55.0	40.9	58.1	58.0	16.9	0.0	2.109061	56940	58119	1162-1290	1.000000	72.5
trnQ-UUG-accD_p2	trnQ-UUG-accD	2	TTCGGAGGTTCGAATCCTTC	ACAGCATAGAATTGAACCACCA	20	22	50.0	40.9	57.3	58.0	16.9	0.0	2.700433	56940	58119	1166-1294	1.000000	67.8
trnQ-UUG-accD_p3	trnQ-UUG-accD	3	GAGGTTCGAATCCTTCCGTC	CAGCATAGAATTGAACCACCA	20	21	55.0	42.9	58.1	56.2	16.9	0.0	2.853245	56940	58119	1161-1289	1.000000	66.6
trnQ-UUG-accD_p4	trnQ-UUG-accD	4	GAGGTTCGAATCCTTCCGTC	ACAGCATAGAATTGAACCACC	20	21	55.0	42.9	58.1	56.2	16.9	0.0	2.855692	56940	58119	1162-1290	1.000000	66.6
trnQ-UUG-accD_p5	trnQ-UUG-accD	5	GGTTTTGGTCCCGTTATTCG	ACAGCATAGAATTGAACCACCA	20	22	50.0	40.9	57.1	58.0	0.0	0.0	2.938919	56940	58119	1182-1310	1.000000	65.8
petA-psbJ_p1	petA-psbJ	1	TGAGAAGGTTCAATTGTCCGA	ACAGGTATTGTCGTGATCGG	21	20	42.9	50.0	57.2	57.4	5.1	0.0	2.341614	63244	64346	1187-1262	1.000000	69.5
petA-psbJ_p2	petA-psbJ	2	TGAGAAGGTTCAATTGTCCGA	CAGGTATTGTCGTGATCGGT	21	20	42.9	50.0	57.2	57.4	5.1	0.0	2.341614	63244	64346	1186-1261	1.000000	69.5
petA-psbJ_p3	petA-psbJ	3	TGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGATCATCC	21	20	42.9	50.0	57.2	57.1	5.1	4.1	2.649558	63244	64346	1138-1213	1.000000	67.2
petA-psbJ_p4	petA-psbJ	4	TGAGAAGGTTCAATTGTCCGAA	ACAGGTATTGTCGTGATCGG	22	20	40.9	50.0	57.9	57.4	5.1	0.0	2.659186	63244	64346	1187-1262	1.000000	66.9
petA-psbJ_p5	petA-psbJ	5	TGAGAAGGTTCAATTGTCCGAA	CAGGTATTGTCGTGATCGGT	22	20	40.9	50.0	57.9	57.4	5.1	0.0	2.659186	63244	64346	1186-1261	1.000000	66.9
rps8-rpl14_p1	rps8-rpl14	1	CAGCGATGGTATCTTTACCCA	GTTTCATTAGCACCCGAGGT	21	20	47.6	50.0	57.5	57.9	0.0	0.0	1.578433	80664	81050	436-513	1.000000	76.6
rps8-rpl14_p2	rps8-rpl14	2	AGCGATGGTATCTTTACCCAC	GTTTCATTAGCACCCGAGGT	21	20	47.6	50.0	57.5	57.9	0.0	0.0	1.580069	80664	81050	435-512	1.000000	76.6
rps8-rpl14_p3	rps8-rpl14	3	CAGCGATGGTATCTTTACCCA	CCCGGGAATTGAGACAGTTC	21	20	47.6	55.0	57.5	58.3	0.0	18.5	1.729942	80664	81050	471-548	1.000000	75.1
rps8-rpl14_p4	rps8-rpl14	4	AGCGATGGTATCTTTACCCAC	CCCGGGAATTGAGACAGTTC	21	20	47.6	55.0	57.5	58.3	0.0	18.5	1.731578	80664	81050	470-547	1.000000	75.1
rps8-rpl14_p5	rps8-rpl14	5	TCTATTCCTGTCAGCATTGCA	GTTTCATTAGCACCCGAGGT	21	20	42.9	50.0	57.4	57.9	11.9	0.0	1.742068	80664	81050	474-551	1.000000	75.1
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	GAGGTTAAGCGTACTCTGGC	ATTCGATCCCGGCATCAAAA	20	20	55.0	45.0	58.1	57.9	0.0	6.1	0.127435	97655	99314	80-1901	1.000000	85.8
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	GAGGTTAAGCGTACTCTGGC	TTCGATCCCGGCATCAAAAT	20	20	55.0	45.0	58.1	57.9	0.0	6.1	0.127435	97655	99314	80-1900	1.000000	85.8
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	GGTTAAGCGTACTCTGGCAA	ATTCGATCCCGGCATCAAAA	20	20	50.0	45.0	57.9	57.9	0.0	6.1	0.140284	97655	99314	80-1899	1.000000	85.7
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	GGTTAAGCGTACTCTGGCAA	TTCGATCCCGGCATCAAAAT	20	20	50.0	45.0	57.9	57.9	0.0	6.1	0.140284	97655	99314	80-1898	1.000000	85.7
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	GAGGTTAAGCGTACTCTGGC	AATTCTCATTCGATCCCGGC	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.191866	97655	99314	80-1908	0.961538	83.0
trnA-UGC_p1	trnA-UGC	1	TGATTTACTTCACGGGCGAG	AGCATAGATCAAACTGCCGA	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.992593	102324	103201	80-1078	1.000000	78.3
trnA-UGC_p2	trnA-UGC	2	TGACTCCTTCATGCATGCTC	AGCATAGATCAAACTGCCGA	20	20	50.0	45.0	58.0	57.0	22.2	0.0	1.022115	102324	103201	80-994	0.961538	76.8
trnA-UGC_p3	trnA-UGC	3	TGATTTACTTCACGGGCGAG	CCGAATCGGCAAAATTGAGT	20	20	50.0	45.0	58.0	57.1	0.0	9.4	0.924249	102324	103201	80-1058	0.923077	71.9
trnA-UGC_p4	trnA-UGC	4	AGGTCTCTGGTTCAAGTCCA	CCGAATCGGCAAAATTGAGT	20	20	50.0	45.0	57.9	57.1	0.0	9.4	1.022820	102324	103201	80-1040	0.923077	71.2
trnA-UGC_p5	trnA-UGC	5	TGACTCCTTCATGCATGCTC	CCGAATCGGCAAAATTGAGT	20	20	50.0	45.0	58.0	57.1	22.2	9.4	0.953770	102324	103201	80-974	0.884615	70.0
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCAAGTCCT	20	20	55.0	50.0	57.8	58.0	18.9	0.0	0.221277	107052	107592	80-638	1.000000	87.4
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.526686	107052	107592	80-681	1.000000	84.4
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	CCGATTGGTCGTAGGTTCAA	20	20	55.0	50.0	57.8	57.6	18.9	0.0	0.589447	107052	107592	80-643	1.000000	84.4
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	GGCCTGTAGCTCAGAGGATT	TGGTCGTAGGTTCAAGTCCT	20	20	55.0	50.0	58.9	58.0	12.9	0.0	0.878027	107052	107592	80-649	1.000000	82.0
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	GTTCCTCAGTAGCTCAGTGG	20	20	55.0	55.0	57.8	57.3	18.9	0.0	0.879733	107052	107592	80-682	1.000000	81.6
ycf1-rps15_p1	ycf1-rps15	1	ACCCATAATGGAAGCCGTTT	TCAACTGATCCGGGGTTTTC	20	20	45.0	50.0	57.5	57.8	0.0	0.0	0.719306	113507	114078	80-790	1.000000	83.2
ycf1-rps15_p2	ycf1-rps15	2	ACCCATAATGGAAGCCGTTT	CAACTGATCCGGGGTTTTCT	20	20	45.0	50.0	57.5	57.8	0.0	0.0	0.725478	113507	114078	80-789	1.000000	83.2
ycf1-rps15_p3	ycf1-rps15	3	ACCCATAATGGAAGCCGTTT	ACTGATCCGGGGTTTTCTTC	20	20	45.0	50.0	57.5	57.5	0.0	0.0	1.016780	113507	114078	80-787	1.000000	80.8
ycf1-rps15_p4	ycf1-rps15	4	ACCCATAATGGAAGCCGTTT	TTCAACTGATCCGGGGTTTT	20	20	45.0	45.0	57.5	57.3	0.0	0.0	1.193249	113507	114078	80-791	1.000000	79.4
ycf1-rps15_p5	ycf1-rps15	5	ACCCATAATGGAAGCCGTTT	CTGATCCGGGGTTTTCTTCT	20	20	45.0	50.0	57.5	57.2	0.0	0.0	1.314514	113507	114078	80-786	1.000000	78.5