Hotspot

ndhF-rpl32

SSC · 116949-117975 · 1027 bp

Back to Glycosmis

Pi 0.0152

MarkerSeek score 63.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1027
variable_sites: 23
pi: 0.0152
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTTTGTTGCGGTCGGAAAAA	CGCTAACGAAAAGGCCTTCA	40.0	50.0	57.4	58.8	1234-1265 bp	1.0	70.9
ndhF-rpl32_p2	TTTTGTTGCGGTCGGAAAAA	GCTAACGAAAAGGCCTTCAAC	40.0	47.6	57.4	58.1	1233-1264 bp	1.0	69.1
ndhF-rpl32_p3	TTTTGTTGCGGTCGGAAAAA	CCTTCAACGCTGCCCAATA	40.0	52.6	57.4	58.1	1220-1251 bp	1.0	68.6
ndhF-rpl32_p4	TTTTGTTGCGGTCGGAAAAA	CTAACGAAAAGGCCTTCAACG	40.0	47.6	57.4	57.8	1232-1263 bp	1.0	68.2
ndhF-rpl32_p5	TTTTGTTGCGGTCGGAAAAA	TAACGAAAAGGCCTTCAACG	40.0	45.0	57.4	56.7	1231-1262 bp	1.0	67.0