Hotspot

rpl20-rps12

LSC · 72926-73698 · 773 bp

Back to Glycosmis

Pi 0.00949

MarkerSeek score 64.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 11
pi: 0.00949
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTAAGTCGTGAATGAGCCCC	CCGAGGAACATGTACAAGGG	55.0	55.0	58.1	58.0	896-913 bp	1.0	80.3
rpl20-rps12_p2	CTAAGTCGTGAATGAGCCCC	AACCCAAGACAGCCAATCAG	55.0	50.0	58.1	58.1	958-975 bp	1.0	79.8
rpl20-rps12_p3	CTAAGTCGTGAATGAGCCCC	CCCAAGACAGCCAATCAGAA	55.0	50.0	58.1	57.8	956-973 bp	1.0	78.8
rpl20-rps12_p4	TTTGTTCTACGTCTCCGAGC	CCGAGGAACATGTACAAGGG	50.0	55.0	57.7	58.0	848-865 bp	1.0	78.1
rpl20-rps12_p5	TGTTCTACGTCTCCGAGCTA	CCGAGGAACATGTACAAGGG	50.0	55.0	57.6	58.0	846-863 bp	1.0	77.7