Hotspot

ndhC-trnM-CAT

LSC · 53667-55468 · 1802 bp

Back to Glycosmis

Pi 0.00407

MarkerSeek score 60.80

Primer available yes

Label ndhC-trnM-CAT

Feature Metadata

label_name: ndhC-trnM-CAT
feature_type: igs
region: LSC
length_bp: 1802
variable_sites: 11
pi: 0.00407
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAT_p1	TCTGGCCCTTTGTTAATCGG	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.9	58.4	1962-1968 bp	1.0	76.3
ndhC-trnM-CAT_p2	TAATCGGGGCTAAAACTGCG	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.3	58.4	1949-1955 bp	1.0	74.5
ndhC-trnM-CAT_p3	TCTGGCCCTTTGTTAATCGG	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.9	57.3	1961-1967 bp	1.0	74.2
ndhC-trnM-CAT_p4	TCTGGCCCTTTGTTAATCGG	ATTAGATACCATCGGCCGTG	50.0	50.0	57.9	57.3	2026-2032 bp	1.0	73.6
ndhC-trnM-CAT_p5	TCTGGCCCTTTGTTAATCGG	CATCGGCCGTGGTATCTAAT	50.0	50.0	57.9	57.3	2017-2023 bp	1.0	73.6