Hotspot

trnT-TGT-trnF-GAA

LSC · 49793-51517 · 1725 bp

Back to Glycosmis

Pi 0.00372

MarkerSeek score 62.10

Primer available yes

Label trnT-TGT-trnF-GAA

Feature Metadata

label_name: trnT-TGT-trnF-GAA
feature_type: igs
region: LSC
length_bp: 1725
variable_sites: 9
pi: 0.00372
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-TGT-trnF-GAA_p1	GCGATGCTCTAACCTCTGAG	TCTACCAGCTGAGCTATCCC	55.0	55.0	57.9	58.0	1764-1778 bp	1.0	80.5
trnT-TGT-trnF-GAA_p2	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	55.0	50.0	57.9	58.1	1800-1814 bp	1.0	80.1
trnT-TGT-trnF-GAA_p3	GGAATCGAACCGATGACCAT	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.8	58.0	1796-1810 bp	1.0	79.7
trnT-TGT-trnF-GAA_p4	GCGATGCTCTAACCTCTGAG	TCAGTCCTCTGCTCTACCAG	55.0	55.0	57.9	57.9	1776-1790 bp	1.0	79.5
trnT-TGT-trnF-GAA_p5	GGAATCGAACCGATGACCAT	TTTGAACTGGTGACACGAGG	50.0	50.0	57.8	58.1	1832-1846 bp	1.0	79.2