Hotspot

atpH-atpI

LSC · 14716-15945 · 1230 bp

Back to Glycosmis

Pi 0.0068

MarkerSeek score 58.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1230
variable_sites: 8
pi: 0.0068
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	927-1364 bp	1.0	83.3
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	880-1317 bp	1.0	83.3
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.9	57.9	983-1420 bp	1.0	82.6
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	936-1373 bp	1.0	82.6
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.9	57.9	965-1402 bp	1.0	82.5