Hotspot

rps16-trnQ-TTG

LSC · 6215-7487 · 1273 bp

Back to Glycosmis

Pi 0.00814

MarkerSeek score 52.80

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 1273
variable_sites: 15
pi: 0.00814
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1338-1361 bp	1.0	74.4
rps16-trnQ-TTG_p2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	1341-1364 bp	1.0	72.2
rps16-trnQ-TTG_p3	CGTTGCTTTCTACCACATCG	GGGTTTTGGTCCCGGTATTC	50.0	55.0	57.2	58.5	1359-1382 bp	1.0	70.7
rps16-trnQ-TTG_p4	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.2	57.3	1342-1365 bp	1.0	69.7
rps16-trnQ-TTG_p5	CGTTGCTTTCTACCACATCG	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.2	58.7	1368-1391 bp	1.0	69.5