primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	trnH-GTG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	75	523	518-566	1.000000	81.9
trnH-GTG-psbA_p2	trnH-GTG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	75	523	532-580	1.000000	81.8
trnH-GTG-psbA_p3	trnH-GTG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	75	523	531-579	1.000000	81.8
trnH-GTG-psbA_p4	trnH-GTG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	75	523	533-581	1.000000	81.8
trnH-GTG-psbA_p5	trnH-GTG-psbA	5	GATCCACTTGGCTACATCCG	ATCGTGCTAACCTTGGGATG	20	20	55.0	50.0	58.1	57.7	0.0	0.0	0.460983	75	523	568-616	1.000000	79.6
rps16-trnQ-TTG_p1	rps16-trnQ-TTG	1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	6215	7487	1338-1361	1.000000	74.4
rps16-trnQ-TTG_p2	rps16-trnQ-TTG	2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	59.1	58.1	0.0	16.9	1.134559	6215	7487	1341-1364	1.000000	72.2
rps16-trnQ-TTG_p3	rps16-trnQ-TTG	3	CGTTGCTTTCTACCACATCG	GGGTTTTGGTCCCGGTATTC	20	20	50.0	55.0	57.2	58.5	0.0	0.0	1.320866	6215	7487	1359-1382	1.000000	70.7
rps16-trnQ-TTG_p4	rps16-trnQ-TTG	4	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	57.2	57.3	0.0	16.9	1.455196	6215	7487	1342-1365	1.000000	69.7
rps16-trnQ-TTG_p5	rps16-trnQ-TTG	5	CGTTGCTTTCTACCACATCG	TAAGGCAACGGGTTTTGGTC	20	20	50.0	50.0	57.2	58.7	0.0	0.0	1.467216	6215	7487	1368-1391	1.000000	69.5
psbI-trnS-GCT_p1	psbI-trnS-GCT	1	CTGTCTAATGATCCAGGGCG	TGAGTGGATTAAAGCGTCGG	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165405	8613	8770	266-277	1.000000	83.7
psbI-trnS-GCT_p2	psbI-trnS-GCT	2	CTGTCTAATGATCCAGGGCG	TAGGAGAGATGGCTGAGTGG	20	20	55.0	55.0	57.8	57.9	0.0	0.0	0.239992	8613	8770	279-290	1.000000	82.9
psbI-trnS-GCT_p3	psbI-trnS-GCT	3	CTGTCTAATGATCCAGGGCG	GGCTGAGTGGATTAAAGCGT	20	20	55.0	50.0	57.8	58.3	0.0	0.0	0.421756	8613	8770	269-280	1.000000	81.6
psbI-trnS-GCT_p4	psbI-trnS-GCT	4	CTGTCTAATGATCCAGGGCG	TGGCTGAGTGGATTAAAGCG	20	20	55.0	50.0	57.8	58.3	0.0	0.0	0.422984	8613	8770	270-281	1.000000	81.6
psbI-trnS-GCT_p5	psbI-trnS-GCT	5	CTGTCTAATGATCCAGGGCG	GAGTGGATTAAAGCGTCGGA	20	20	55.0	50.0	57.8	57.7	0.0	0.0	0.449783	8613	8770	265-276	1.000000	81.5
trnS-GCT-trnR-TCT_p1	trnS-GCT-trnR-TCT	1	CCGACGCTTTAATCCACTCA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.056187	8859	10723	1932-1957	1.000000	68.9
trnS-GCT-trnR-TCT_p2	trnS-GCT-trnR-TCT	2	CCGACGCTTTAATCCACTCA	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.139515	8859	10723	1938-1963	1.000000	68.2
trnS-GCT-trnR-TCT_p3	trnS-GCT-trnR-TCT	3	ACGCTTTAATCCACTCAGCC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.3	56.9	0.0	0.0	2.312538	8859	10723	1929-1954	1.000000	66.9
trnS-GCT-trnR-TCT_p4	trnS-GCT-trnR-TCT	4	CGCTTTAATCCACTCAGCCA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.3	56.9	0.0	0.0	2.313766	8859	10723	1928-1953	1.000000	66.8
trnS-GCT-trnR-TCT_p5	trnS-GCT-trnR-TCT	5	TCCGACGCTTTAATCCACTC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	57.7	56.9	0.0	0.0	2.340565	8859	10723	1933-1958	1.000000	66.6
trnR-TCT-atpA_p1	trnR-TCT-atpA	1	ATGAAAGGCGTCCATTGTCT	TCAGGAACAAAAGGAGCGTT	20	20	45.0	45.0	57.5	57.7	0.0	0.0	0.838745	10796	11053	372-397	1.000000	77.3
trnR-TCT-atpA_p2	trnR-TCT-atpA	2	ATGAAAGGCGTCCATTGTCT	TTCAGGAACAAAAGGAGCGT	20	20	45.0	45.0	57.5	57.7	0.0	0.0	0.838745	10796	11053	373-398	1.000000	77.2
trnR-TCT-atpA_p3	trnR-TCT-atpA	3	AATGGGAATGAAAGGCGTCC	TCAGGAACAAAAGGAGCGTT	20	20	50.0	45.0	58.5	57.7	0.0	0.0	0.867517	10796	11053	379-404	1.000000	77.0
trnR-TCT-atpA_p4	trnR-TCT-atpA	4	GGGAATGAAAGGCGTCCATT	TCAGGAACAAAAGGAGCGTT	20	20	50.0	45.0	58.5	57.7	6.7	0.0	0.867517	10796	11053	376-401	1.000000	77.0
trnR-TCT-atpA_p5	trnR-TCT-atpA	5	GGGAATGAAAGGCGTCCATT	TTCAGGAACAAAAGGAGCGT	20	20	50.0	45.0	58.5	57.7	6.7	0.0	0.867517	10796	11053	377-402	1.000000	77.0
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	20	20	45.0	50.0	58.0	57.9	1.4	0.0	0.167157	14716	15945	927-1364	1.000000	83.3
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	14716	15945	880-1317	1.000000	83.3
atpH-atpI_p3	atpH-atpI	3	TACCTTGACCAACTCCAGGT	CCCGTCATGTTCCTTGGATT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.241050	14716	15945	983-1420	1.000000	82.6
atpH-atpI_p4	atpH-atpI	4	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.245019	14716	15945	936-1373	1.000000	82.6
atpH-atpI_p5	atpH-atpI	5	GTCCAATAGAAGCAAGCCCA	CCCGTCATGTTCCTTGGATT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.257541	14716	15945	965-1402	1.000000	82.5
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ATTCCCGCATTTCCATCTCC	ACCAGCCAAGCAGAATAAGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.147907	28256	29482	1371-1413	1.000000	79.8
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ATTCCCGCATTTCCATCTCC	TCCTTTGTTGATCAGGCGAC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.204707	28256	29482	1337-1379	1.000000	79.4
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ATTCCCGCATTTCCATCTCC	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.0	58.4	0.0	14.6	0.368609	28256	29482	1297-1339	1.000000	78.1
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ATTCCCGCATTTCCATCTCC	CAGCCAAGCAGAATAAGGGA	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.438261	28256	29482	1369-1411	1.000000	77.5
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	ATTCCCGCATTTCCATCTCC	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.489360	28256	29482	1322-1364	1.000000	77.1
trnD-GTC-trnY-GTA_p1	trnD-GTC-trnY-GTA	1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.716274	33130	33602	539-546	1.000000	76.2
trnD-GTC-trnY-GTA_p2	trnD-GTC-trnY-GTA	2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.717825	33130	33602	538-545	1.000000	76.2
trnD-GTC-trnY-GTA_p3	trnD-GTC-trnY-GTA	3	CGGTGCTCTGACCAATTGAA	TCTTCTTCTTCCTGGGTCGA	20	20	50.0	50.0	58.5	57.7	7.1	0.0	0.781553	33130	33602	596-603	1.000000	75.5
trnD-GTC-trnY-GTA_p4	trnD-GTC-trnY-GTA	4	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	33130	33602	560-567	1.000000	75.2
trnD-GTC-trnY-GTA_p5	trnD-GTC-trnY-GTA	5	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.5	57.6	7.1	0.0	0.852505	33130	33602	559-566	1.000000	75.0
trnT-TGT-trnF-GAA_p1	trnT-TGT-trnF-GAA	1	GCGATGCTCTAACCTCTGAG	TCTACCAGCTGAGCTATCCC	20	20	55.0	55.0	57.9	58.0	0.0	17.4	0.135246	49793	51517	1764-1778	1.000000	80.5
trnT-TGT-trnF-GAA_p2	trnT-TGT-trnF-GAA	2	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	57.9	58.1	0.0	20.1	0.184636	49793	51517	1800-1814	1.000000	80.1
trnT-TGT-trnF-GAA_p3	trnT-TGT-trnF-GAA	3	GGAATCGAACCGATGACCAT	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	57.8	58.0	15.5	17.4	0.242999	49793	51517	1796-1810	1.000000	79.7
trnT-TGT-trnF-GAA_p4	trnT-TGT-trnF-GAA	4	GCGATGCTCTAACCTCTGAG	TCAGTCCTCTGCTCTACCAG	20	20	55.0	55.0	57.9	57.9	0.0	0.0	0.269038	49793	51517	1776-1790	1.000000	79.5
trnT-TGT-trnF-GAA_p5	trnT-TGT-trnF-GAA	5	GGAATCGAACCGATGACCAT	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.8	58.1	15.5	20.1	0.292389	49793	51517	1832-1846	1.000000	79.2
ndhC-trnM-CAT_p1	ndhC-trnM-CAT	1	TCTGGCCCTTTGTTAATCGG	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.534109	53667	55468	1962-1968	1.000000	76.3
ndhC-trnM-CAT_p2	ndhC-trnM-CAT	2	TAATCGGGGCTAAAACTGCG	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	58.3	58.4	0.0	0.0	0.752332	53667	55468	1949-1955	1.000000	74.5
ndhC-trnM-CAT_p3	ndhC-trnM-CAT	3	TCTGGCCCTTTGTTAATCGG	CCGCCGTATGAAAGCAATAC	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.786062	53667	55468	1961-1967	1.000000	74.2
ndhC-trnM-CAT_p4	ndhC-trnM-CAT	4	TCTGGCCCTTTGTTAATCGG	ATTAGATACCATCGGCCGTG	20	20	50.0	50.0	57.9	57.3	0.0	27.1	0.866419	53667	55468	2026-2032	1.000000	73.6
ndhC-trnM-CAT_p5	ndhC-trnM-CAT	5	TCTGGCCCTTTGTTAATCGG	CATCGGCCGTGGTATCTAAT	20	20	50.0	50.0	57.9	57.3	0.0	27.1	0.866419	53667	55468	2017-2023	1.000000	73.6
accD-psaI_p1	accD-psaI	1	TGTTCCATGGCTGTTTTCCT	AGAGGGAAAGCTGTTGAGAA	20	20	45.0	45.0	57.6	56.4	7.0	0.0	2.008928	61921	62704	838-847	1.000000	66.0
accD-psaI_p2	accD-psaI	2	AAAAGGTGTTCTGAGCGAGT	AGAGGGAAAGCTGTTGAGAA	20	20	45.0	45.0	57.4	56.4	0.0	0.0	2.250927	61921	62704	864-873	1.000000	64.0
accD-psaI_p3	accD-psaI	3	AAAGGTGTTCTGAGCGAGTT	AGAGGGAAAGCTGTTGAGAA	20	20	45.0	45.0	57.4	56.4	0.0	0.0	2.250927	61921	62704	863-872	1.000000	64.0
accD-psaI_p4	accD-psaI	4	CTGTTCCATGGCTGTTTTCC	AGAGGGAAAGCTGTTGAGAA	20	20	50.0	45.0	57.3	56.4	7.0	0.0	2.359413	61921	62704	839-848	1.000000	63.2
accD-psaI_p5	accD-psaI	5	TGTTCCATGGCTGTTTTCCT	AGAGGGAAAGCTGTTGAGAAT	20	21	45.0	42.9	57.6	56.9	7.0	0.0	2.466337	61921	62704	838-847	1.000000	62.4
ycf4-cemA_p1	ycf4-cemA	1	TGACTCCGCGAGAAATTGAG	ATGAGAGAGGGATCCACCAG	20	20	50.0	55.0	58.0	57.9	8.2	15.2	0.103836	63808	64575	899-927	1.000000	82.9
ycf4-cemA_p2	ycf4-cemA	2	TGAGAATTTGACTCCGCGAG	ATGAGAGAGGGATCCACCAG	20	20	50.0	55.0	58.0	57.9	8.2	15.2	0.103836	63808	64575	907-935	1.000000	82.9
ycf4-cemA_p3	ycf4-cemA	3	TTGGCCTATTTCTTGCGTGT	ATGAGAGAGGGATCCACCAG	20	20	45.0	55.0	58.1	57.9	3.3	15.2	0.195841	63808	64575	864-892	1.000000	82.4
ycf4-cemA_p4	ycf4-cemA	4	TGACTCCGCGAGAAATTGAG	TAAGGGGAATGGATGCGTTC	20	20	50.0	50.0	58.0	57.7	8.2	10.5	0.345555	63808	64575	842-870	1.000000	81.3
ycf4-cemA_p5	ycf4-cemA	5	TGAGAATTTGACTCCGCGAG	TAAGGGGAATGGATGCGTTC	20	20	50.0	50.0	58.0	57.7	8.2	10.5	0.345555	63808	64575	850-878	1.000000	81.2
psbJ-psbL_p1	psbJ-psbL	1	TCCTTCCAGTAGTATCGGCC	TGAACCGTACCAGTCTCTACT	20	21	55.0	47.6	58.3	57.6	0.0	0.0	1.729087	67706	67847	244-244	1.000000	67.2
psbJ-psbL_p2	psbJ-psbL	2	TCCTTCCAGTAGTATCGGCC	TGACACAATCAAACCCAAACG	20	21	55.0	42.9	58.3	57.6	0.0	0.0	1.727791	67706	67847	280-280	1.000000	67.0
psbJ-psbL_p3	psbJ-psbL	3	TCCTTCCAGTAGTATCGGCC	GACACAATCAAACCCAAACGA	20	21	55.0	42.9	58.3	57.3	0.0	0.0	2.000576	67706	67847	279-279	1.000000	64.9
psbJ-psbL_p4	psbJ-psbL	4	CCTTCCAGTAGTATCGGCCA	TGAACCGTACCAGTCTCTACT	20	21	55.0	47.6	58.6	57.6	2.2	0.0	2.020038	67706	67847	243-243	1.000000	64.8
psbJ-psbL_p5	psbJ-psbL	5	CCTTCCAGTAGTATCGGCCA	TGACACAATCAAACCCAAACG	20	21	55.0	42.9	58.6	57.6	2.2	0.0	2.018741	67706	67847	279-279	1.000000	64.7
rpl20-rps12_p1	rpl20-rps12	1	CTAAGTCGTGAATGAGCCCC	CCGAGGAACATGTACAAGGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.082851	72926	73698	896-913	1.000000	80.3
rpl20-rps12_p2	rpl20-rps12	2	CTAAGTCGTGAATGAGCCCC	AACCCAAGACAGCCAATCAG	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.143534	72926	73698	958-975	1.000000	79.8
rpl20-rps12_p3	rpl20-rps12	3	CTAAGTCGTGAATGAGCCCC	CCCAAGACAGCCAATCAGAA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.263697	72926	73698	956-973	1.000000	78.8
rpl20-rps12_p4	rpl20-rps12	4	TTTGTTCTACGTCTCCGAGC	CCGAGGAACATGTACAAGGG	20	20	50.0	55.0	57.7	58.0	0.0	0.0	0.367085	72926	73698	848-865	1.000000	78.1
rpl20-rps12_p5	rpl20-rps12	5	TGTTCTACGTCTCCGAGCTA	CCGAGGAACATGTACAAGGG	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.415791	72926	73698	846-863	1.000000	77.7
ndhF-rpl32_p1	ndhF-rpl32	1	TTTTGTTGCGGTCGGAAAAA	CGCTAACGAAAAGGCCTTCA	20	20	40.0	50.0	57.4	58.8	0.0	20.8	1.495978	116949	117975	1234-1265	1.000000	70.9
ndhF-rpl32_p2	ndhF-rpl32	2	TTTTGTTGCGGTCGGAAAAA	GCTAACGAAAAGGCCTTCAAC	20	21	40.0	47.6	57.4	58.1	0.0	20.8	1.719371	116949	117975	1233-1264	1.000000	69.1
ndhF-rpl32_p3	ndhF-rpl32	3	TTTTGTTGCGGTCGGAAAAA	CCTTCAACGCTGCCCAATA	20	19	40.0	52.6	57.4	58.1	0.0	0.0	1.782459	116949	117975	1220-1251	1.000000	68.6
ndhF-rpl32_p4	ndhF-rpl32	4	TTTTGTTGCGGTCGGAAAAA	CTAACGAAAAGGCCTTCAACG	20	21	40.0	47.6	57.4	57.8	0.0	20.8	1.832567	116949	117975	1232-1263	1.000000	68.2
ndhF-rpl32_p5	ndhF-rpl32	5	TTTTGTTGCGGTCGGAAAAA	TAACGAAAAGGCCTTCAACG	20	20	40.0	45.0	57.4	56.7	0.0	20.8	1.982641	116949	117975	1231-1262	1.000000	67.0
rpl32-trnL-TAG_p1	rpl32-trnL-TAG	1	AGACAGAGATAAGGGCGGAA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.168551	118135	118872	1289-1346	1.000000	85.2
rpl32-trnL-TAG_p2	rpl32-trnL-TAG	2	AGACAGAGATAAGGGCGGAA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.169921	118135	118872	1290-1347	1.000000	85.2
rpl32-trnL-TAG_p3	rpl32-trnL-TAG	3	CGAAATCCCTTTCTACCGGG	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	58.1	58.0	6.3	0.0	0.058365	118135	118872	823-823	0.333333	52.9
rpl32-trnL-TAG_p4	rpl32-trnL-TAG	4	CGAAATCCCTTTCTACCGGG	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	58.1	58.0	6.3	0.0	0.059735	118135	118872	824-824	0.333333	52.9
rpl32-trnL-TAG_p5	rpl32-trnL-TAG	5	CGAAATCCCTTTCTACCGGG	GCTGCATAGCCCAATACCAT	20	20	55.0	50.0	58.1	58.1	6.3	0.0	0.135394	118135	118872	1425-1425	0.333333	52.3
ndhA_p1	ndhA	1	ACCGGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	20	20	50.0	50.0	58.0	57.9	12.9	0.0	0.173319	124555	126771	2325-2341	1.000000	80.2
ndhA_p2	ndhA	2	ACCGGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	20	20	50.0	50.0	58.0	57.8	12.9	0.0	0.238750	124555	126771	2323-2339	1.000000	79.7
ndhA_p3	ndhA	3	ACCGGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	20	20	50.0	50.0	58.0	58.5	12.9	6.3	0.563760	124555	126771	2423-2439	1.000000	77.0
ndhA_p4	ndhA	4	ACCGGTTACCATAGGGAACA	TGGGGGAAGTTGATCGTTGA	20	20	50.0	50.0	58.0	58.9	12.9	0.0	0.983820	124555	126771	2324-2340	1.000000	73.7
ndhA_p5	ndhA	5	ACCGGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAA	20	19	50.0	52.6	58.0	58.0	12.9	6.3	1.080206	124555	126771	2423-2439	1.000000	72.9