Hotspot

rpl20

LSC · 67514-67906 · 393 bp

Back to Glycine

Pi 0.0161

MarkerSeek score 40.50

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 393
variable_sites: 17
pi: 0.0161
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	AGTCGACCGCTAGAACTACT	TGGATGGGTAGAGCCAAAGA	50.0	50.0	57.6	58.0	1054-1077 bp	1.0	81.9
rpl20_p2	AGTCGACCGCTAGAACTACT	GGACCTGCCCGTTTCTAAAA	50.0	50.0	57.6	58.1	959-977 bp	1.0	81.7
rpl20_p3	AGTCGACCGCTAGAACTACT	AAGAAAGAAAGGCTCCGGTG	50.0	50.0	57.6	58.1	987-1005 bp	1.0	81.6
rpl20_p4	AGTCGACCGCTAGAACTACT	ACGGAATAAGAGGGTCAGCT	50.0	50.0	57.6	58.1	1156-1179 bp	1.0	80.8
rpl20_p5	AGTCGACCGCTAGAACTACT	GGACGAACGGAATAAGAGGG	50.0	55.0	57.6	57.8	1162-1183 bp	0.727273	67.4