Hotspot

ndhF

SSC · 124726-126987 · 2262 bp

Back to Glycine

Pi 0.0157

MarkerSeek score 52.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2262
variable_sites: 97
pi: 0.0157
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AACCGAACCCCTTCTTTACA	TAGGCTTGTGGAAGAAGGGA	45.0	50.0	56.7	58.0	2652-2681 bp	1.0	72.4
ndhF_p2	ACCGAACCCCTTCTTTACAA	TAGGCTTGTGGAAGAAGGGA	45.0	50.0	56.7	58.0	2651-2680 bp	1.0	72.4
ndhF_p3	AACCGAACCCCTTCTTTACA	CCTTCTTCGAGCTAGGCTTG	45.0	55.0	56.7	58.1	2664-2693 bp	1.0	72.1
ndhF_p4	ACCGAACCCCTTCTTTACAA	CCTTCTTCGAGCTAGGCTTG	45.0	55.0	56.7	58.1	2663-2692 bp	1.0	72.1
ndhF_p5	ACCGAACCCCTTCTTTACAA	GGGCCCTCTTATCTCTTCCT	45.0	55.0	56.7	57.9	2680-2709 bp	1.0	71.7