Hotspot

ndhD-ccsA

SSC · 122465-122722 · 258 bp

Back to Glycine

Pi 0.0364

MarkerSeek score 68.40

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 258
variable_sites: 26
pi: 0.0364
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TTTCGGCTGACAAGGTTGAA	AGTTATGGTTCATTTACACCT	45.0	33.3	58.0	52.4	328-340 bp	1.0	47.9
ndhD-ccsA_p2	TTTCGGCTGACAAGGTTGAA	AGGACTACATAGTTATGGTTCA	45.0	36.4	58.0	53.7	338-350 bp	1.0	47.7
ndhD-ccsA_p3	GGCTGACAAGGTTGAAGCTA	AGGACTACATAGTTATGGTTCA	50.0	36.4	57.8	53.7	334-346 bp	1.0	47.7
ndhD-ccsA_p4	TTTTCGGCTGACAAGGTTGA	AGGACTACATAGTTATGGTTCA	45.0	36.4	58.0	53.7	339-351 bp	1.0	47.6
ndhD-ccsA_p5	TCATTTTCGGCTGACAAGGT	AGGACTACATAGTTATGGTTCA	45.0	36.4	57.7	53.7	342-354 bp	1.0	47.6