Hotspot

rps18-rpl20

LSC · 67294-67513 · 220 bp

Back to Glycine

Pi 0.046

MarkerSeek score 67.30

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 25
pi: 0.046
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	GATCGCTTGTGTGTTACCCT	ATGCAGTAATTCGCGGGAAT	50.0	45.0	57.9	58.0	947-973 bp	1.0	87.7
rps18-rpl20_p2	GCTTGTGTGTTACCCTTCGA	ATGCAGTAATTCGCGGGAAT	50.0	45.0	58.1	58.0	943-969 bp	1.0	87.5
rps18-rpl20_p3	GATCGCTTGTGTGTTACCCT	GCTTTCGAGGAGCTCATTCA	50.0	50.0	57.9	58.0	1076-1102 bp	1.0	86.9
rps18-rpl20_p4	GCTTGTGTGTTACCCTTCGA	GCTTTCGAGGAGCTCATTCA	50.0	50.0	58.1	58.0	1072-1098 bp	1.0	86.7
rps18-rpl20_p5	TCGCTTGTGTGTTACCCTTC	GCTTTCGAGGAGCTCATTCA	50.0	50.0	58.1	58.0	1074-1100 bp	1.0	86.7