Hotspot

petA-psbJ

LSC · 62262-63151 · 890 bp

Back to Glycine

Pi 0.0236

MarkerSeek score 70.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 890
variable_sites: 58
pi: 0.0236
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	CATATTCCGGATTGGGCTCA	42.9	50.0	57.2	57.4	734-957 bp	1.0	70.7
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGCTCATCT	42.9	50.0	57.2	58.9	731-954 bp	1.0	68.4
petA-psbJ_p3	TGAGAAGGTTCAATTGTCCGA	TATTCCGGATTGGGCTCATC	42.9	50.0	57.2	57.1	732-955 bp	1.0	68.4
petA-psbJ_p4	TGAGAAGGTTCAATTGTCCGA	TCATATTCCGGATTGGGCTC	42.9	50.0	57.2	57.1	735-958 bp	1.0	68.4
petA-psbJ_p5	TGAGAAGGTTCAATTGTCCGAA	CATATTCCGGATTGGGCTCA	40.9	50.0	57.9	57.4	734-957 bp	1.0	68.2