Hotspot

trnQ-UUG-rps16

LSC · 55205-55687 · 483 bp

Back to Glycine

Pi 0.0307

MarkerSeek score 67.20

Primer available yes

Label trnQ-UUG-rps16

Feature Metadata

label_name: trnQ-UUG-rps16
feature_type: igs
region: LSC
length_bp: 483
variable_sites: 42
pi: 0.0307
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-rps16_p1	GAGGTTCGAATCCTTCCGTC	GTCTTTCAAGTCGCACGTTG	55.0	50.0	58.1	58.0	561-584 bp	1.0	86.5
trnQ-UUG-rps16_p2	GAGGTTCGAATCCTTCCGTC	ATGTCTTTCAAGTCGCACGT	55.0	45.0	58.1	58.1	563-586 bp	1.0	85.5
trnQ-UUG-rps16_p3	GAGGTTCGAATCCTTCCGTC	GCACGTTGCTTTTTACCACA	55.0	45.0	58.1	57.8	549-572 bp	1.0	85.2
trnQ-UUG-rps16_p4	GAGGTTCGAATCCTTCCGTC	CATGTCTTTCAAGTCGCACG	55.0	50.0	58.1	57.8	564-587 bp	1.0	84.8
trnQ-UUG-rps16_p5	GAGGTTCGAATCCTTCCGTC	GGCGGATGTAAGAATCCACA	55.0	50.0	58.1	57.7	595-618 bp	1.0	83.8