Hotspot

trnG-GCC-psbZ

LSC · 24526-25234 · 709 bp

Back to Glycine

Pi 0.0228

MarkerSeek score 68.20

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 51
pi: 0.0228
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGAAGGTTGGTCG	50.0	55.0	57.8	57.9	684-900 bp	1.0	87.0
trnG-GCC-psbZ_p2	CGTCTTCTCCTTGGCAAAGA	TCTCCTGAAGGTTGGTCGAA	50.0	50.0	57.8	58.3	682-898 bp	1.0	85.4
trnG-GCC-psbZ_p3	CGTCTTCTCCTTGGCAAAGA	TTCTCCTGAAGGTTGGTCGA	50.0	50.0	57.8	58.3	683-899 bp	1.0	85.4
trnG-GCC-psbZ_p4	CGTCTTCTCCTTGGCAAAGA	CTCCTGAAGGTTGGTCGAAT	50.0	50.0	57.8	57.2	681-897 bp	1.0	81.5
trnG-GCC-psbZ_p5	CGGATAGCGGGAATCGAAC	CTTCTCCTGAAGGTTGGTCG	57.9	55.0	58.2	57.9	706-922 bp	1.0	78.7