Hotspot

ndhJ-trnF-GAA

LSC · 12391-13082 · 692 bp

Back to Glycine

Pi 0.0258

MarkerSeek score 64.30

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 53
pi: 0.0258
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	TGAAATACAAGATGCTCACT	CCTCGTGTCACCAGTTCAAA	35.0	50.0	52.2	58.1	742-750 bp	1.0	47.1
ndhJ-trnF-GAA_p2	TGAAATACAAGATGCTCACT	AGAATGGTCGGGATAGCTCA	35.0	50.0	52.2	57.9	787-795 bp	1.0	46.7
ndhJ-trnF-GAA_p3	TGAAATACAAGATGCTCACT	AATGGTCGGGATAGCTCAGT	35.0	50.0	52.2	58.2	785-793 bp	1.0	46.7
ndhJ-trnF-GAA_p4	TGAAATACAAGATGCTCACT	ATGGTCGGGATAGCTCAGTT	35.0	50.0	52.2	58.2	784-792 bp	1.0	46.7
ndhJ-trnF-GAA_p5	TGAAATACAAGATGCTCACT	TATGCGTCAAGAATGGTCGG	35.0	50.0	52.2	58.1	796-804 bp	1.0	46.6