Hotspot

trnK-UUU-rbcL

LSC · 4272-5302 · 1031 bp

Back to Glycine

Pi 0.0279

MarkerSeek score 72.70

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 1031
variable_sites: 83
pi: 0.0279
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AGAGTCGCTCTTAAAAGCCG	TTGAAGCATGTGTGAAGGCT	50.0	45.0	58.0	58.0	1230-1261 bp	1.0	89.8
trnK-UUU-rbcL_p2	AGTCGCTCTTAAAAGCCGAG	TTGAAGCATGTGTGAAGGCT	50.0	45.0	58.0	58.0	1228-1259 bp	1.0	89.8
trnK-UUU-rbcL_p3	GAGTCGCTCTTAAAAGCCGA	TTGAAGCATGTGTGAAGGCT	50.0	45.0	58.0	58.0	1229-1260 bp	1.0	89.8
trnK-UUU-rbcL_p4	AGAGTCGCTCTTAAAAGCCG	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.0	58.0	1147-1178 bp	1.0	89.8
trnK-UUU-rbcL_p5	AGTCGCTCTTAAAAGCCGAG	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.0	58.0	1145-1176 bp	1.0	89.8