Hotspot

trnL-trnF

LSC · 47996-48359 · 364 bp

Back to Glyceria

Pi 0.0203

MarkerSeek score 70.00

Primer available yes

Label trnL-trnF

Feature Metadata

label_name: trnL-trnF
feature_type: igs
region: LSC
length_bp: 364
variable_sites: 34
pi: 0.0203
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnF_p1	AAGTCGTGAGGGTTCAAGTC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	428-470 bp	1.0	85.6
trnL-trnF_p2	AAGTCGTGAGGGTTCAAGTC	CTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	57.8	443-485 bp	1.0	84.2
trnL-trnF_p3	AAGTCGTGAGGGTTCAAGTC	TCTGCCAGGAACCAGATTTG	50.0	50.0	57.5	57.8	444-486 bp	1.0	84.1
trnL-trnF_p4	AAGTCGTGAGGGTTCAAGTC	TTTTCTCTGCCAGGAACCAG	50.0	50.0	57.5	57.7	449-491 bp	1.0	83.5
trnL-trnF_p5	AAGTCGTGAGGGTTCAAGTC	TCTCTGCCAGGAACCAGATT	50.0	50.0	57.5	58.3	446-488 bp	1.0	83.0