Hotspot

ycf3-rps4

LSC · 44667-45646 · 980 bp

Back to Glyceria

Pi 0.0219

MarkerSeek score 68.40

Primer available yes

Label ycf3-rps4

Feature Metadata

label_name: ycf3-rps4
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 69
pi: 0.0219
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-rps4_p1	TTTTCCCCTGAAGTTGTCGG	ATAGGAAGTGGGTCGGTCTC	50.0	55.0	58.0	58.2	1096-1165 bp	1.0	87.0
ycf3-rps4_p2	TTTTCCCCTGAAGTTGTCGG	GATAGGAAGTGGGTCGGTCT	50.0	55.0	58.0	58.2	1097-1166 bp	1.0	87.0
ycf3-rps4_p3	TTTTCCCCTGAAGTTGTCGG	GAAGTGGGTCGGTCTCAAAA	50.0	50.0	58.0	57.8	1092-1161 bp	1.0	86.8
ycf3-rps4_p4	CCCCTGAAGTTGTCGGAATT	ATAGGAAGTGGGTCGGTCTC	50.0	55.0	57.8	58.2	1092-1161 bp	1.0	85.6
ycf3-rps4_p5	CCCCTGAAGTTGTCGGAATT	GATAGGAAGTGGGTCGGTCT	50.0	55.0	57.8	58.2	1093-1162 bp	1.0	85.6