Hotspot

trnG-trnT

LSC · 13749-15387 · 1639 bp

Back to Glyceria

Pi 0.0246

MarkerSeek score 74.50

Primer available yes

Label trnG-trnT

Feature Metadata

label_name: trnG-trnT
feature_type: igs
region: LSC
length_bp: 1639
variable_sites: 164
pi: 0.0246
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnT_p1	ACGAATCACACTTTTACCACT	ACGTCTTACCACGGCATTAC	38.1	50.0	55.5	58.0	989-1802 bp	0.966667	62.0
trnG-trnT_p2	AGAACGAATCACACTTTTACCAC	ACGTCTTACCACGGCATTAC	39.1	50.0	57.3	58.0	992-1805 bp	0.966667	60.9
trnG-trnT_p3	GAACGAATCACACTTTTACCACT	ACGTCTTACCACGGCATTAC	39.1	50.0	57.3	58.0	991-1804 bp	0.966667	60.9
trnG-trnT_p4	AACGAATCACACTTTTACCACT	ACGTCTTACCACGGCATTAC	36.4	50.0	56.2	58.0	990-1803 bp	0.966667	60.0
trnG-trnT_p5	ACGAATCACACTTTTACCACT	CGTCTTACCACGGCATTACT	38.1	50.0	55.5	57.7	988-1801 bp	0.966667	59.7