Hotspot

psbK-psbI

LSC · 7168-7566 · 399 bp

Back to Glyceria

Pi 0.0233

MarkerSeek score 65.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 42
pi: 0.0233
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGTCCTGGGTCATTGGATA	50.0	50.0	57.9	58.1	484-525 bp	1.0	89.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGTCCTGGGTCATTGGAT	50.0	50.0	57.9	58.1	485-526 bp	1.0	89.4
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCACGCCCAG	50.0	50.0	57.9	57.8	508-549 bp	1.0	88.7
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	TTACGTCCTGGGTCATTGGA	50.0	50.0	57.9	58.4	486-527 bp	1.0	87.6
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	ACGTCCTGGGTCATTGGATA	45.0	50.0	58.3	58.1	481-522 bp	1.0	87.4