Hotspot

rps16-trnQ

LSC · 5527-6597 · 1071 bp

Back to Glyceria

Pi 0.0188

MarkerSeek score 72.80

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1071
variable_sites: 91
pi: 0.0188
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	AGTGTCGCATAAAACCCGAA	45.0	45.0	58.1	57.8	838-1247 bp	0.966667	85.9
rps16-trnQ_p2	TCATGTCCTTCAAGTCGCAC	AGTGTCGCATAAAACCCGAA	50.0	45.0	58.2	57.8	849-1258 bp	0.966667	85.3
rps16-trnQ_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCAGCGGGTTTTGATC	45.0	50.0	58.1	58.5	799-1208 bp	1.0	84.8
rps16-trnQ_p4	TCATGTCCTTCAAGTCGCAC	TAAGGCAGCGGGTTTTGATC	50.0	50.0	58.2	58.5	810-1219 bp	1.0	84.3
rps16-trnQ_p5	ATCATGTCCTTCAAGTCGCA	AGTGTCGCATAAAACCCGAA	45.0	45.0	57.2	57.8	850-1259 bp	0.966667	80.8