primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnK_p1 trnK 1 AACTCAGATTGCTCTTTTCT TCGATCGTATCTAAGATCCT 20 20 35.0 40.0 52.4 52.2 0.0 15.9 11.409023 1383 3947 2594-2642 0.900000 41.4 trnK_p2 trnK 2 AACTCAGATTGCTCTTTTCT TTCGATCGTATCTAAGATCCT 20 21 35.0 38.1 52.4 53.2 0.0 15.9 11.454008 1383 3947 2595-2643 0.900000 41.4 trnK_p3 trnK 3 AAACTCAGATTGCTCTTTTCT TCGATCGTATCTAAGATCCT 21 20 33.3 40.0 53.3 52.2 0.0 15.9 11.460168 1383 3947 2595-2643 0.900000 41.4 trnK_p4 trnK 4 AAACTCAGATTGCTCTTTTCT TTCGATCGTATCTAAGATCCT 21 21 33.3 38.1 53.3 53.2 0.0 15.9 11.505153 1383 3947 2596-2644 0.900000 41.4 trnK_p5 trnK 5 AACTCAGATTGCTCTTTTCT TTTCGATCGTATCTAAGATCCT 20 22 35.0 36.4 52.4 54.0 0.0 15.9 11.585436 1383 3947 2596-2644 0.900000 41.4 trnK-rps16_p1 trnK-rps16 1 TGAGGATCAGTCGTGGTCTT TCGAGCCGTATGAGGAGAAA 20 20 50.0 50.0 58.1 58.2 0.0 0.0 0.316333 3948 4493 940-990 1.000000 86.9 trnK-rps16_p2 trnK-rps16 2 TGAGGATCAGTCGTGGTCTT TTTCTCGAGCCGTATGAGGA 20 20 50.0 50.0 58.1 58.2 0.0 7.8 0.316333 3948 4493 944-994 1.000000 86.9 trnK-rps16_p3 trnK-rps16 3 ACGTCTAATTAGAGCGGGGA TCGAGCCGTATGAGGAGAAA 20 20 50.0 50.0 57.9 58.2 26.5 0.0 0.304034 3948 4493 1127-1176 1.000000 86.7 trnK-rps16_p4 trnK-rps16 4 ACGTCTAATTAGAGCGGGGA TTTCTCGAGCCGTATGAGGA 20 20 50.0 50.0 57.9 58.2 26.5 7.8 0.304034 3948 4493 1131-1180 1.000000 86.7 trnK-rps16_p5 trnK-rps16 5 ACGTCTAATTAGAGCGGGGA CCTTTGAAGTCTGTCAGGCA 20 20 50.0 50.0 57.9 57.7 26.5 7.3 0.323814 3948 4493 1259-1308 1.000000 85.9 rps16-trnQ_p1 rps16-trnQ 1 AAGTCGCACGTTGCTTTCTA AGTGTCGCATAAAACCCGAA 20 20 45.0 45.0 58.1 57.8 8.1 0.0 0.312175 5527 6597 838-1247 0.966667 85.9 rps16-trnQ_p2 rps16-trnQ 2 TCATGTCCTTCAAGTCGCAC AGTGTCGCATAAAACCCGAA 20 20 50.0 45.0 58.2 57.8 0.0 0.0 0.377084 5527 6597 849-1258 0.966667 85.3 rps16-trnQ_p3 rps16-trnQ 3 AAGTCGCACGTTGCTTTCTA TAAGGCAGCGGGTTTTGATC 20 20 45.0 50.0 58.1 58.5 8.1 0.0 0.679874 5527 6597 799-1208 1.000000 84.8 rps16-trnQ_p4 rps16-trnQ 4 TCATGTCCTTCAAGTCGCAC TAAGGCAGCGGGTTTTGATC 20 20 50.0 50.0 58.2 58.5 0.0 0.0 0.744782 5527 6597 810-1219 1.000000 84.3 rps16-trnQ_p5 rps16-trnQ 5 ATCATGTCCTTCAAGTCGCA AGTGTCGCATAAAACCCGAA 20 20 45.0 45.0 57.2 57.8 0.0 0.0 0.941104 5527 6597 850-1259 0.966667 80.8 psbK-psbI_p1 psbK-psbI 1 GTTTGGCAAGCTGCTGTAAG ACGTCCTGGGTCATTGGATA 20 20 50.0 50.0 57.9 58.1 1.4 0.0 0.181965 7168 7566 484-525 1.000000 89.4 psbK-psbI_p2 psbK-psbI 2 GTTTGGCAAGCTGCTGTAAG TACGTCCTGGGTCATTGGAT 20 20 50.0 50.0 57.9 58.1 1.4 0.0 0.181965 7168 7566 485-526 1.000000 89.4 psbK-psbI_p3 psbK-psbI 3 GTTTGGCAAGCTGCTGTAAG TTTTTACTCCTCACGCCCAG 20 20 50.0 50.0 57.9 57.8 1.4 0.0 0.235006 7168 7566 508-549 1.000000 88.7 psbK-psbI_p4 psbK-psbI 4 GTTTGGCAAGCTGCTGTAAG TTACGTCCTGGGTCATTGGA 20 20 50.0 50.0 57.9 58.4 1.4 0.0 0.409182 7168 7566 486-527 1.000000 87.6 psbK-psbI_p5 psbK-psbI 5 TGGCAAGCTGCTGTAAGTTT ACGTCCTGGGTCATTGGATA 20 20 45.0 50.0 58.3 58.1 1.4 0.0 0.434674 7168 7566 481-522 1.000000 87.4 trnS-psbD_p1 trnS-psbD 1 ATTAGCAATCCGCCGCTTTA GACAAAAACGAAACGGTCCC 20 20 45.0 50.0 58.0 57.9 0.0 0.0 0.147815 7886 8842 1042-1115 1.000000 87.6 trnS-psbD_p2 trnS-psbD 2 ATTAGCAATCCGCCGCTTTA ACAAAAACGAAACGGTCCCT 20 20 45.0 45.0 58.0 58.2 0.0 0.0 0.284276 7886 8842 1041-1114 1.000000 86.6 trnS-psbD_p3 trnS-psbD 3 ATTAGCAATCCGCCGCTTTA CCGACAAAAACGAAACGGTC 20 20 45.0 50.0 58.0 58.3 0.0 0.0 0.304501 7886 8842 1044-1117 1.000000 86.4 trnS-psbD_p4 trnS-psbD 4 ATTAGCAATCCGCCGCTTTA CGACAAAAACGAAACGGTCC 20 20 45.0 50.0 58.0 58.3 0.0 0.0 0.304501 7886 8842 1043-1116 1.000000 86.4 trnS-psbD_p5 trnS-psbD 5 GAGCGGAAAGAGAGGGATTC GACAAAAACGAAACGGTCCC 20 20 55.0 50.0 57.8 57.9 0.0 0.0 0.342365 7886 8842 1093-1166 1.000000 85.9 trnG-trnfM_p1 trnG-trnfM 1 AGGAGAAGATACGGGTTCGA CGAGGCTCATAACCTTGAGG 20 20 50.0 55.0 57.6 57.8 0.0 16.0 0.663168 12397 12861 539-768 1.000000 84.1 trnG-trnfM_p2 trnG-trnfM 2 AGGAGAAGATACGGGTTCGA TTCCACCTTTTTCGTCCACC 20 20 50.0 50.0 57.6 58.3 0.0 0.0 0.749214 12397 12861 602-832 1.000000 83.8 trnG-trnfM_p3 trnG-trnfM 3 AGGAGAAGATACGGGTTCGA CACCTTTTTCGTCCACCAGT 20 20 50.0 50.0 57.6 58.3 0.0 0.0 0.756402 12397 12861 599-829 1.000000 83.8 trnG-trnfM_p4 trnG-trnfM 4 AGGAGAAGATACGGGTTCGA CAGTTTGGTAGCTCACGAGG 20 20 50.0 55.0 57.6 58.3 0.0 0.0 0.717159 12397 12861 554-783 1.000000 83.5 trnG-trnfM_p5 trnG-trnfM 5 AGGAGAAGATACGGGTTCGA TTTTTCGTCCACCAGTTCCC 20 20 50.0 50.0 57.6 58.3 0.0 0.0 0.749214 12397 12861 595-825 0.900000 79.0 trnG-trnT_p1 trnG-trnT 1 ACGAATCACACTTTTACCACT ACGTCTTACCACGGCATTAC 21 20 38.1 50.0 55.5 58.0 0.0 0.0 3.536272 13749 15387 989-1802 0.966667 62.0 trnG-trnT_p2 trnG-trnT 2 AGAACGAATCACACTTTTACCAC ACGTCTTACCACGGCATTAC 23 20 39.1 50.0 57.3 58.0 0.0 0.0 3.668987 13749 15387 992-1805 0.966667 60.9 trnG-trnT_p3 trnG-trnT 3 GAACGAATCACACTTTTACCACT ACGTCTTACCACGGCATTAC 23 20 39.1 50.0 57.3 58.0 0.0 0.0 3.668987 13749 15387 991-1804 0.966667 60.9 trnG-trnT_p4 trnG-trnT 4 AACGAATCACACTTTTACCACT ACGTCTTACCACGGCATTAC 22 20 36.4 50.0 56.2 58.0 0.0 0.0 3.776743 13749 15387 990-1803 0.966667 60.0 trnG-trnT_p5 trnG-trnT 5 ACGAATCACACTTTTACCACT CGTCTTACCACGGCATTACT 21 20 38.1 50.0 55.5 57.7 0.0 0.0 3.824803 13749 15387 988-1801 0.966667 59.7 trnC-rpoB_p1 trnC-rpoB 1 TTATTGATAAGGCGGCACCC CCGGGTATTGTGGACATTCC 20 20 50.0 55.0 58.0 58.3 13.1 0.0 0.348788 19124 20237 1230-1314 1.000000 82.7 trnC-rpoB_p2 trnC-rpoB 2 TTATTGATAAGGCGGCACCC CGGGTATTGTGGACATTCCC 20 20 50.0 55.0 58.0 58.3 13.1 0.0 0.348788 19124 20237 1229-1313 1.000000 82.7 trnC-rpoB_p3 trnC-rpoB 3 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 20 20 50.0 55.0 58.1 58.3 14.6 0.0 0.395580 19124 20237 1193-1277 1.000000 82.5 trnC-rpoB_p4 trnC-rpoB 4 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 20 20 50.0 55.0 58.1 58.3 14.6 0.0 0.395580 19124 20237 1192-1276 1.000000 82.5 trnC-rpoB_p5 trnC-rpoB 5 TTATTGATAAGGCGGCACCC AAGTTCTTCTGCCAAGCCTT 20 20 50.0 45.0 58.0 57.6 13.1 0.0 0.395998 19124 20237 1294-1378 1.000000 82.1 ycf3-rps4_p1 ycf3-rps4 1 TTTTCCCCTGAAGTTGTCGG ATAGGAAGTGGGTCGGTCTC 20 20 50.0 55.0 58.0 58.2 0.0 0.0 0.246400 44667 45646 1096-1165 1.000000 87.0 ycf3-rps4_p2 ycf3-rps4 2 TTTTCCCCTGAAGTTGTCGG GATAGGAAGTGGGTCGGTCT 20 20 50.0 55.0 58.0 58.2 0.0 0.0 0.246400 44667 45646 1097-1166 1.000000 87.0 ycf3-rps4_p3 ycf3-rps4 3 TTTTCCCCTGAAGTTGTCGG GAAGTGGGTCGGTCTCAAAA 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.274184 44667 45646 1092-1161 1.000000 86.8 ycf3-rps4_p4 ycf3-rps4 4 CCCCTGAAGTTGTCGGAATT ATAGGAAGTGGGTCGGTCTC 20 20 50.0 55.0 57.8 58.2 0.0 0.0 0.422432 44667 45646 1092-1161 1.000000 85.6 ycf3-rps4_p5 ycf3-rps4 5 CCCCTGAAGTTGTCGGAATT GATAGGAAGTGGGTCGGTCT 20 20 50.0 55.0 57.8 58.2 0.0 0.0 0.422432 44667 45646 1093-1162 1.000000 85.6 trnT-trnL_p1 trnT-trnL 1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.032006 46628 47405 833-919 1.000000 90.5 trnT-trnL_p2 trnT-trnL 2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.073242 46628 47405 825-911 1.000000 90.2 trnT-trnL_p3 trnT-trnL 3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.140570 46628 47405 803-889 1.000000 89.8 trnT-trnL_p4 trnT-trnL 4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 20 20 50.0 55.0 58.0 58.2 0.0 10.2 0.272624 46628 47405 823-909 1.000000 88.6 trnT-trnL_p5 trnT-trnL 5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 20 20 50.0 50.0 58.0 57.7 0.0 0.0 0.303758 46628 47405 832-918 1.000000 88.3 trnL-trnF_p1 trnL-trnF 1 AAGTCGTGAGGGTTCAAGTC TTTGAACTGGTGACACGAGG 20 20 50.0 50.0 57.5 58.1 0.0 20.1 0.579937 47996 48359 428-470 1.000000 85.6 trnL-trnF_p2 trnL-trnF 2 AAGTCGTGAGGGTTCAAGTC CTGCCAGGAACCAGATTTGA 20 20 50.0 50.0 57.5 57.8 0.0 0.0 0.733063 47996 48359 443-485 1.000000 84.2 trnL-trnF_p3 trnL-trnF 3 AAGTCGTGAGGGTTCAAGTC TCTGCCAGGAACCAGATTTG 20 20 50.0 50.0 57.5 57.8 0.0 0.0 0.733063 47996 48359 444-486 1.000000 84.1 trnL-trnF_p4 trnL-trnF 4 AAGTCGTGAGGGTTCAAGTC TTTTCTCTGCCAGGAACCAG 20 20 50.0 50.0 57.5 57.7 0.0 0.0 0.804773 47996 48359 449-491 1.000000 83.5 trnL-trnF_p5 trnL-trnF 5 AAGTCGTGAGGGTTCAAGTC TCTCTGCCAGGAACCAGATT 20 20 50.0 50.0 57.5 58.3 0.0 0.0 0.866578 47996 48359 446-488 1.000000 83.0 trnF-ndhJ_p1 trnF-ndhJ 1 CCTCGTGTCACCAGTTCAAA CCTAATGCCCGAAAGTTGGA 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.174679 48433 48920 606-644 1.000000 86.2 trnF-ndhJ_p2 trnF-ndhJ 2 CCTCGTGTCACCAGTTCAAA TCCTAATGCCCGAAAGTTGG 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.174679 48433 48920 607-645 1.000000 86.1 trnF-ndhJ_p3 trnF-ndhJ 3 CCTCGTGTCACCAGTTCAAA ATAGGCTGGCCCTTACGTAA 20 20 50.0 50.0 58.1 58.2 0.0 7.9 0.254575 48433 48920 587-625 1.000000 85.7 trnF-ndhJ_p4 trnF-ndhJ 4 CCTCGTGTCACCAGTTCAAA TCCACGCCTTAAACGTATCC 20 20 50.0 50.0 58.1 57.7 0.0 3.2 0.350307 48433 48920 624-662 1.000000 84.7 trnF-ndhJ_p5 trnF-ndhJ 5 CCTCGTGTCACCAGTTCAAA CCACGCCTTAAACGTATCCT 20 20 50.0 50.0 58.1 57.7 0.0 3.2 0.356647 48433 48920 623-661 1.000000 84.6 ndhC-trnV_p1 ndhC-trnV 1 CTAACCGGGGCTAAAAGTCC GGTTCGAATCCGTATAGCCC 20 20 55.0 55.0 58.0 57.9 6.3 0.0 0.110083 50592 51451 882-1020 0.933333 85.2 ndhC-trnV_p2 ndhC-trnV 2 CTAACCGGGGCTAAAAGTCC CGGTTCGAATCCGTATAGCC 20 20 55.0 55.0 58.0 58.3 6.3 22.8 0.339465 50592 51451 883-1021 0.933333 83.4 ndhC-trnV_p3 ndhC-trnV 3 TAACCGGGGCTAAAAGTCCT GGTTCGAATCCGTATAGCCC 20 20 50.0 55.0 58.3 57.9 6.3 0.0 0.415737 50592 51451 881-1019 0.933333 82.8 ndhC-trnV_p4 ndhC-trnV 4 CACTAACCGGGGCTAAAAGT GGTTCGAATCCGTATAGCCC 20 20 50.0 55.0 57.5 57.9 6.3 0.0 0.560721 50592 51451 884-1022 0.933333 81.6 ndhC-trnV_p5 ndhC-trnV 5 TAACCGGGGCTAAAAGTCCT CGGTTCGAATCCGTATAGCC 20 20 50.0 55.0 58.3 58.3 6.3 22.8 0.645119 50592 51451 882-1020 0.933333 80.9 rbcL-psaI_p1 rbcL-psaI 1 TCGAGTTCGAGCCAGTAGAT CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.0 58.0 0.0 26.9 0.066821 56607 58039 1407-1697 1.000000 88.7 rbcL-psaI_p2 rbcL-psaI 2 TCGAGTTCGAGCCAGTAGAT AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 58.0 57.9 0.0 26.8 0.143355 56607 58039 1413-1703 1.000000 88.1 rbcL-psaI_p3 rbcL-psaI 3 AATGGAGTCCTGAACTTGCC CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 57.8 58.0 11.3 26.9 0.233579 56607 58039 1458-1748 1.000000 87.2 rbcL-psaI_p4 rbcL-psaI 4 CGCAGCTTGTGAAGTATGGA CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.3 58.0 0.0 26.9 0.301363 56607 58039 1439-1729 1.000000 86.7 rbcL-psaI_p5 rbcL-psaI 5 AATGGAGTCCTGAACTTGCC AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 57.8 57.9 11.3 26.8 0.310113 56607 58039 1464-1754 1.000000 86.6 psbE-petL_p1 psbE-petL 1 TGACAAATAACCAACCCGCA AAGCAGCCAGTAGAAAACCG 20 20 45.0 50.0 58.0 58.5 0.0 0.0 0.493582 62733 63935 1361-1440 1.000000 82.1 psbE-petL_p2 psbE-petL 2 TGACAAATAACCAACCCGCA GCAGCCAGTAGAAAACCGAA 20 20 45.0 50.0 58.0 58.5 0.0 0.0 0.497931 62733 63935 1359-1438 1.000000 82.0 psbE-petL_p3 psbE-petL 3 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCG 20 20 50.0 50.0 57.9 58.5 6.4 0.0 0.527577 62733 63935 1350-1429 1.000000 81.8 psbE-petL_p4 psbE-petL 4 CAACCCGCAATGAATAGGGA GCAGCCAGTAGAAAACCGAA 20 20 50.0 50.0 57.9 58.5 6.4 0.0 0.531926 62733 63935 1348-1427 1.000000 81.8 psbE-petL_p5 psbE-petL 5 ACCAACCCGCAATGAATAGG AAGCAGCCAGTAGAAAACCG 20 20 50.0 50.0 58.2 58.5 0.0 0.0 0.713535 62733 63935 1352-1431 1.000000 80.4 ndhF_p1 ndhF 1 AACACCCATCAATGCATGGT TAAGCAAGAGCCGCATCTTT 20 20 45.0 45.0 58.1 57.9 0.0 0.0 0.172483 102384 104603 2761-2824 0.433333 60.0 ndhF_p2 ndhF 2 ACCCATCAATGCATGGTGTT TAAGCAAGAGCCGCATCTTT 20 20 45.0 45.0 58.1 57.9 0.0 0.0 0.172483 102384 104603 2758-2821 0.433333 60.0 ndhF_p3 ndhF 3 AATCGCGGAAAACCGAAGTA TAAGCAAGAGCCGCATCTTT 20 20 45.0 45.0 57.9 57.9 6.1 0.0 0.197027 102384 104603 2655-2718 0.433333 60.0 ndhF_p4 ndhF 4 ATCGCGGAAAACCGAAGTAA TAAGCAAGAGCCGCATCTTT 20 20 45.0 45.0 57.9 57.9 6.1 0.0 0.197027 102384 104603 2654-2717 0.433333 60.0 ndhF_p5 ndhF 5 ACCCATCAATGCATGGTGTT CCTATAAGCAAGAGCCGCAT 20 20 45.0 50.0 58.1 57.8 0.0 0.0 0.234500 102384 104603 2762-2825 0.433333 59.4 ndhF-rpl32_p1 ndhF-rpl32 1 ACACATTCCCACAAGTTCCC GTTGCGCAGAAAAACCCTTT 20 20 50.0 45.0 58.0 58.1 0.0 11.3 0.070035 104604 105425 1423-1614 1.000000 90.0 ndhF-rpl32_p2 ndhF-rpl32 2 ACACATTCCCACAAGTTCCC TTGCGCAGAAAAACCCTTTG 20 20 50.0 45.0 58.0 58.1 0.0 11.3 0.071377 104604 105425 1422-1613 1.000000 90.0 ndhF-rpl32_p3 ndhF-rpl32 3 CACATTCCCACAAGTTCCCA GTTGCGCAGAAAAACCCTTT 20 20 50.0 45.0 58.0 58.1 0.0 11.3 0.071455 104604 105425 1422-1613 1.000000 90.0 ndhF-rpl32_p4 ndhF-rpl32 4 TACGTGAAGAGGGAATTGCG GTTGCGCAGAAAAACCCTTT 20 20 50.0 45.0 58.0 58.1 0.0 11.3 0.069994 104604 105425 1696-1887 1.000000 89.5 ndhF-rpl32_p5 ndhF-rpl32 5 TACGTGAAGAGGGAATTGCG TTGCGCAGAAAAACCCTTTG 20 20 50.0 45.0 58.0 58.1 0.0 11.3 0.071336 104604 105425 1695-1886 1.000000 89.5 rpl32-trnL_p1 rpl32-trnL 1 GGATAAGACCCCGTACCGTA TTCGAACCGAGATGCTTGAG 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.022452 105618 106259 1141-1271 0.966667 89.8 rpl32-trnL_p2 rpl32-trnL 2 GGATAAGACCCCGTACCGTA GGATTCGAACCGAGATGCTT 20 20 55.0 50.0 58.0 58.1 0.0 0.0 0.077419 105618 106259 1144-1274 0.966667 89.4 rpl32-trnL_p3 rpl32-trnL 3 GGATAAGACCCCGTACCGTA GATCCGCACAAAAGAGTTGC 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.049640 105618 106259 1693-1823 0.966667 88.0 rpl32-trnL_p4 rpl32-trnL 4 AAAGGGTTTTTCTGCGCAAC TTCGAACCGAGATGCTTGAG 20 20 45.0 50.0 58.1 58.0 13.1 0.0 0.069120 105618 106259 699-869 0.700000 77.8 rpl32-trnL_p5 rpl32-trnL 5 CAAAGGGTTTTTCTGCGCAA TTCGAACCGAGATGCTTGAG 20 20 45.0 50.0 58.1 58.0 13.1 0.0 0.070462 105618 106259 700-870 0.700000 77.7