Hotspot

rps16

LSC · 5484-6627 · 1144 bp

Back to Glochidion

Pi 0.00219

MarkerSeek score 47.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1144
variable_sites: 5
pi: 0.00219
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGGGGATAGTAGAGAAAAGGT	CTGGATTACTATGTACCCGCG	47.6	52.4	55.8	58.1	1309-1315 bp	1.0	46.5
rps16_p2	GGGGGATAGTAGAGAAAAGGT	TGGATTACTATGTACCCGCG	47.6	50.0	55.8	56.9	1308-1314 bp	1.0	46.2
rps16_p3	GGGGGATAGTAGAGAAAAGGTT	CTGGATTACTATGTACCCGCG	45.5	52.4	56.6	58.1	1309-1315 bp	1.0	44.6
rps16_p4	GGGGGATAGTAGAGAAAAGGTT	TGGATTACTATGTACCCGCG	45.5	50.0	56.6	56.9	1308-1314 bp	1.0	44.3
rps16_p5	GGGGGATAGTAGAGAAAAGGT	GGATTACTATGTACCCGCGA	47.6	50.0	55.8	56.6	1307-1313 bp	1.0	43.9