Hotspot

psbC

LSC · 34528-35949 · 1422 bp

Back to Glochidion

Pi 0.000352

MarkerSeek score 40.40

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 1
pi: 0.000352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GCGGCAGAAGATCCTGAATT	TGAGTTGAAAAGCAAGACTCT	50.0	38.1	58.3	55.4	1621 bp	1.0	48.4
psbC_p2	AGGTATTCGTGCTTGGATGG	TGAGTTGAAAAGCAAGACTCT	50.0	38.1	57.7	55.4	1565 bp	1.0	48.4
psbC_p3	GCGGCAGAAGATCCTGAATT	TGAGTTGAAAAGCAAGACTCTT	50.0	36.4	58.3	56.1	1621 bp	1.0	46.6
psbC_p4	TTTCTCAGGAAATCCGTGCG	TGAGTTGAAAAGCAAGACTCT	50.0	38.1	58.6	55.4	1638 bp	1.0	46.6
psbC_p5	AGGTATTCGTGCTTGGATGG	TGAGTTGAAAAGCAAGACTCTT	50.0	36.4	57.7	56.1	1565 bp	1.0	46.5