Hotspot

ndhA

SSC · 122027-124276 · 2250 bp

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Pi 0.00163

MarkerSeek score 62.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2250
variable_sites: 7
pi: 0.00163
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCCGGTTACCATAGGAAACA	TGGGAGAGGTTGATCGTTGA	45.0	50.0	55.9	58.4	2316-2390 bp	1.0	62.7
ndhA_p2	TCCGGTTACCATAGGAAACA	ATGGGAGAGGTTGATCGTTG	45.0	50.0	55.9	57.3	2317-2391 bp	1.0	60.0
ndhA_p3	TCCGGTTACCATAGGAAACA	GGGAGAGGTTGATCGTTGAA	45.0	50.0	55.9	57.2	2315-2389 bp	1.0	59.5
ndhA_p4	ATCCGGTTACCATAGGAAACA	TGGGAGAGGTTGATCGTTGA	42.9	50.0	56.4	58.4	2317-2391 bp	1.0	59.2
ndhA_p5	TCCGGTTACCATAGGAAACAT	TGGGAGAGGTTGATCGTTGA	42.9	50.0	56.4	58.4	2316-2390 bp	1.0	59.2