Hotspot

trnL-CAA-ndhB

IRb · 95400-95966 · 567 bp

Back to Glochidion

Pi 0.000882

MarkerSeek score 40.30

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 567
variable_sites: 1
pi: 0.000882
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	CAGGAATCTCAATGAACCCGA	50.0	47.6	58.0	57.7	652 bp	1.0	69.9
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	CAGGAATCTCAATGAACCCGA	50.0	47.6	58.0	57.7	650 bp	1.0	69.9
trnL-CAA-ndhB_p3	TACCATTTCACCACCAAGGC	CAGGAATCTCAATGAACCCGA	50.0	47.6	58.1	57.7	640 bp	1.0	69.3
trnL-CAA-ndhB_p4	GTCTCGCGTGTCTACCATTT	ACCAGGAATCTCAATGAACCC	50.0	47.6	58.0	57.6	654 bp	1.0	69.1
trnL-CAA-ndhB_p5	CTCGCGTGTCTACCATTTCA	ACCAGGAATCTCAATGAACCC	50.0	47.6	58.0	57.6	652 bp	1.0	69.1