Hotspot

psbE-petL

LSC · 65351-66424 · 1074 bp

Back to Glochidion

Pi 0.000933

MarkerSeek score 62.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1074
variable_sites: 2
pi: 0.000933
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGGTCAGACC	50.0	50.0	57.8	56.4	1165-1190 bp	1.0	67.7
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGTCGTATCTTGGTCAGACC	55.0	50.0	58.2	56.4	1160-1185 bp	1.0	67.4
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AGTCGTATCTTGGTCAGACC	50.0	50.0	58.4	56.4	1158-1183 bp	1.0	66.2
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGTCGTATCTTGGTCAGACC	50.0	47.6	57.8	57.1	1166-1191 bp	1.0	65.7
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	AAGTCGTATCTTGGTCAGACC	55.0	47.6	58.2	57.1	1161-1186 bp	1.0	65.4