Hotspot

rbcL-accD

LSC · 57028-57801 · 774 bp

Back to Glochidion

Pi 0.00237

MarkerSeek score 61.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 3
pi: 0.00237
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	GGGGTCTTCACTTACACTGG	55.0	55.0	58.0	57.5	974-1009 bp	1.0	76.9
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	GGGGTCTTCACTTACACTGG	55.0	55.0	57.7	57.5	960-995 bp	1.0	74.9
rbcL-accD_p3	GGCTAGCAAATGGAGTCCTG	GGGGTCTTCACTTACACTGG	55.0	55.0	58.3	57.5	972-1007 bp	1.0	74.6
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	AGAGGACCAAGACTATCCGT	55.0	50.0	58.0	57.2	946-981 bp	1.0	74.1
rbcL-accD_p5	GTGAGGCTAGCAAATGGAGT	GGGGTCTTCACTTACACTGG	50.0	55.0	57.6	57.5	976-1011 bp	1.0	74.0